来自Lactobacillus pentosus的外聚糖YY-112:对人类肠道微生物群的结构和影响
Ying Yang1, Mingzhe Wang1, Hui Zhang2
1Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. YangYing_SPS@163.com.
Food & function
|August 7, 2023
概括
一种来自Lactobacillus pentosus的新型益生菌LPE-2通过减少有害气体和增加有益的短链脂肪酸,有效调节肠道微生物群. 这种多糖体显示出潜在的性别特异性益生菌效应,特别是在女性身上.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 营养科学 营养科学
背景情况:
- 新型益生菌对于管理肠道微生物群和预防相关疾病至关重要.
- 乳杆菌托斯YY-112产生一种同质的多糖体,LPE-2,具有潜在的前生物特性.
研究的目的:
- 为了描述 LPE-2 的结构,LPE-2 是来自 Lactobacillus pentosus YY-112.12 的多糖体.
- 为了研究LPE-2对模拟肠道发酵的体外益生菌作用.
- 探索LPE-2对人类肠道菌群的潜在性别特异性影响.
主要方法:
- 使用甲基化,气色谱-质谱法 (GC-MS) 和核磁共振 (NMR) 来分析LPE-2的结构.
- 实验室模拟肠道发酵试验以评估气体和短链脂肪酸 (SCFA) 产量.
- 16S rRNA基因测序以分析肠道细菌的相对丰富性.
主要成果:
- LPE-2是一种分支曼南和线性葡萄糖多糖化物.
- LPE-2发酵减少了有害的肠道气体,增加了SCFA的产生,特别是酸.
- LPE-2降低了埃舍里希亚-希格拉菌的数量,增加了Bifidobacterium和Lactobacillus的数量.
- 在模拟的肠道环境中,LPE-2的益生菌作用更为明显,代表了女性的肠道菌群.
结论:
- LPE-2表现出显著的益生菌活性,调节肠道微生物群的组成和代谢输出.
- LPE-2的独特结构特征,包括曼南分支和α配置的脊柱,可能有助于其被有益细菌选择性地利用.
- LPE-2 显示出作为一种特定于性别的益生菌来增强人类肠道健康的潜力.
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