在马类尿液中检测兴奋剂和基本药物,使用液体染色学-质谱法
Luis Flores1, Sabine Hargrave1, Amel Clifford1
1KL Maddy Equine Analytical Chemistry Laboratory, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
Drug testing and analysis
|August 7, 2023
概括
这项研究开发了一种新方法,在马尿中检测出50多种禁止使用的兴奋剂,包括和小分子. 经过验证的液体色谱-质谱技术为增强的马类药物测试提供了敏感的检测.
科学领域:
- 分析化学 分析化学
- 法医科学 法医科学 法医科学
- 马体育医学 马体育医学
背景情况:
- 赛马中的兴奋剂涉及禁止的药物,如和小分子.
- 由于这些物质的特性和快速消除,检测这些物质是具有挑战性的.
- 敏感的分析技术对于有效的监管至关重要.
研究的目的:
- 开发和验证一种检测马类尿液中50多种兴奋剂的方法.
- 为了应对识别,分泌剂和四级药物的挑战.
- 为反兴奋剂实验室建立一个可靠的选程序.
主要方法:
- 使用混合模式弱阴离子交换吸附剂进行固体相提取.
- 液体染色学-质谱学 (LC-MS) 分析.
- 在三级四极质谱仪上使用选择性反应监测进行检测.
主要成果:
- 这种方法能够检测50多种不同的基于的兴奋剂,分泌剂和四级药物.
- 检测极限一般在ng/mL以下的范围内.
- 足够的精度,准确性和稳定性用于初始测试程序.
结论:
- 开发的LC-MS方法适用于在马类尿液中检测广泛的具有挑战性的兴奋剂.
- 该方法为反兴奋剂查提供了敏感和可靠的结果.
- 经过验证的参数证实了该方法在药物测试实验室常规使用中的有效性.
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