来自Boletus的多糖化合物的结构特征和体外发酵模式
Li Chen1, Yuxin Wang1, Jiaxin Liu1
1Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, 510632, China. tianlinmin@163.com.
Food & function
|August 7, 2023
概括
波列图斯的多糖化合物被表征并在体外发酵. 它们促进了有益的肠道细菌和SCFA的产生,其中B. bicolor对肠道健康最有前途.
科学领域:
- 菌类学 菌类学是指菌类学.
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- ,特别是Boletus物种,是已知的生物活性化合物的来源.
- 多糖是复杂的碳水化合物,具有潜在的健康益处,包括益生菌效应.
- 了解多糖的结构和发酵对于它们在功能性食品中的应用至关重要.
研究的目的:
- 研究来自Boletus auripes,B. bicolor和B. griseus的多糖的结构特征.
- 评估这些博莱特斯多糖的体外发酵模式和肠道微生物群调节.
- 为了识别具有优越的前生物潜力的特定的Boletus物种.
主要方法:
- 使用亚临界水提取方法,从博莱果实体中分离出多糖.
- 分析了物理化学特性,包括单糖化合物组成和分子量.
- 使用人类便微生物群的模拟体外发酵模型被用来评估多糖的利用率和微生物群落的转移.
主要成果:
- 波列图斯多糖主要由葡萄糖,银河糖和曼诺糖组成.
- 观察到广泛的分子重量 (5 kDa到2000 kDa),在物种之间有所不同.
- 试管发酵证明了多糖的逐渐利用,导致SCFA的产生和肠道微生物群组成的变化.
- 有益的细菌属 (如 Bacteroides, Faecalibacterium) 增加,而有害的属 (如 Sutterella, Escherichia-Shigella) 减少.
- 与其他测试物种相比,来自B. bicolor的多糖体表现出增强的发酵性和益生菌效应.
结论:
- 波列图斯是结构多样化的多糖的宝贵来源.
- 这些多糖类可由肠道微生物群发酵,产生有益的SCFA.
- 博莱图斯多糖化物调节肠道微生物群的组成,促进有益的细菌.
- 博莱图斯双色多糖体显示出作为支持肠道健康的益生菌的巨大潜力.
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