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lncRNA GHET1通过EZH2 / LSD1介导的MT2A表观遗传抑制在孕前中调节异常性热囊细胞表型

Pengyun Wan¹, Jia Huang², Wenting Liu¹

¹Department of Obstetrics and Gynecology, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, People's Republic of China.

Molecular reproduction and development

|August 7, 2023

概括

长非编码RNAGHET1在妊娠前 (PE) 中降低,并促进 trofhoblast 功能. 在PE中,GHET1通过表观遗传抑制MT2A来调节异常热细胞表型.

科学领域:

产科和妇科产科和妇科
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

孕前 (PE) 是一种严重的妊娠并发症,其特征是新发高血压和器官损伤.
长非编码RNAs (lncRNAs) 在PE病变发生中的作用是一个新兴的研究领域.
胃癌高表达转录1 (GHET1) 是一种 lncRNA,在细胞过程中具有潜在的作用.

研究的目的:

研究 lncRNA GHET1 在妊娠前 (PE) 中的作用和潜在机制.
为了确定GHET1对外小细胞 (EVT) 功能的影响.
在PE中阐明涉及GHET1,EZH2/LSD1和MT2A的分子相互作用.

主要方法:

定量实时聚合酶连锁反应 (qRT-PCR) 来评估GHET1的表达.
在体外测试 (CCK-8,殖民地形成,EDU,流细胞计,伤口愈合,Transwell,管形成) 以评估EVT的增殖,细胞循环,迁移,入侵和网络形成.
分子测试 (RIP,RNA pull-down,ChIP) 用于确认相互作用和表观遗传修饰.

主要成果:

在PE患者的胎盘中,GHET1的表达显著下降.
在体外,GHET1过度表达促进了EVT增殖,细胞周期进展,迁移,入侵和网络形成.
GHET1与EZH2和LSD1直接相互作用,通过MT2A促进体的H3K27me3和H3K4me2修饰,导致Metallothionein 2A (MT2A) 的表观遗传抑制.
MT2A的淘汰扭转了GHET1对EVT功能的影响.

结论:

GHET1在调节异常多细胞表型方面发挥着至关重要的作用.
通过EZH2/LSD1复合体,GHET1通过表观遗传下调MT2A来作为PE中的瘤抑制剂.
向GHET1可能为预先孕提供一种潜在的治疗策略.

关键词:

在EZH2/LSD1中使用.在 GHET1 中,GHET1 是 GHET1 的代码.在 MT2A 中,我们可以使用 MT2A.孕前症孕前症是什么在 lncRNA 中,

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