暴露于高剂量的金纳米颗粒通过调节核糖体蛋白表达来加速生长速度
Shabina Quadir1, Nuha Abeer Khan1, Deepak Kumar Singh1
1Multidisciplinary Centre for Advance Research & Studies, Jamia Millia Islamia, New Delhi 110025, India.
ACS nano
|August 7, 2023
概括
高度的金纳米颗粒 (AuNPs) 通过影响翻译机制来加速微生物生长. 这项研究揭示了AuNPs调节核糖体组装,导致更快的细胞增殖,而不会诱导DNA损伤或一般压力.
科学领域:
- 纳米技术纳米技术
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 黄金纳米粒子 (AuNPs) 在生物医学领域使用,但其细胞效应尚未完全理解.
- 研究微生物对AuNPs的反应,为纳米粒子与细胞相互作用提供了洞察力.
研究的目的:
- 探索金纳米粒子 (AuNPs) 对模型微生物的细胞和代谢影响.
- 描述不同AuNP度对细菌和真菌生长和生理学的影响.
主要方法:
- 使用大肠杆菌,细菌和Saccharomyces cerevisiae作为模型生物.
- 合成和表征酸盐覆盖的球形金纳米粒子 (5和10纳米).
- 分析了包括生长率,细胞大小,FtsZ蛋白表达和整个蛋白质组在内的细胞反应.
主要成果:
- 高度的AuNP (10^12颗粒) 减少了细胞分裂时间,增加了细胞密度.
- 暴露的细胞显示平均大小减少和FtsZ表达增加,表明增长加速.
- 没有观察到DNA损伤,外应激或一般应激反应;蛋白质组分析显示调节的核糖体蛋白质表达.
结论:
- 高度的AuNP会对翻译机制产生压力,增强蛋白质合成并加速细胞增殖.
- 加速生长与核糖体组合调节有关,对核糖体向药物敏感.
- 金纳米颗粒可以通过对蛋白质合成的影响影响微生物代谢和生长动态.
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