低温抑制PpCBFs降解pectin,延长桃子的储存时间
Tingting Guo1,2, Jianzhao Li1, Meiling Guo1,2
1The Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, China.
Journal of food science
|August 7, 2023
概括
低温储存通过抑制pectin降解来保持桃子的坚硬性. 这是通过激活桃子C-重复结合因子 (PpCBFs) 来实现的,这些因子抑制关键的素降解酶.
科学领域:
- 植物生理学 植物生理学
- 收获后的生物学
- 分子遗传学 分子遗传学
背景情况:
- 低温储存对于保持桃子水果质量至关重要.
- 低温影响桃子软化的精确分子机制,特别是关于pectin降解和转录因子的精确分子机制,仍然不完全理解.
研究的目的:
- 调查桃子C重复结合因子 (PpCBFs) 在低温储存期间调节点降解和水果软化中的作用.
- 为了阐明不同储存温度下桃子水果的生理和生化变化.
主要方法:
- 对在低温 (LT,6°C) 和室温 (RT,25°C) 保存的桃子进行比较分析.
- 测量水果坚硬度,pectin分数 (水溶性和离子溶性),以及pectin降解酶的活性 (多甲氨酸酶,pectate lyase,pectin methylesterase).
- 对PpPME3,PpPL2,PpPG,PpCBF2和PpCBF3的基因表达分析,以及对DER动机的促进体分析.
主要成果:
- 与室温相比,低温储存显著抑制了桃花的软化,并保持了细胞壁的稳定性.
- 在RT中观察到水溶性和离子溶性pectin的较高水平,而LT则抑制了PG,PL和PME的活动.
- PpCBF2和PpCBF3的表达在LT上升调节,并且与水果坚硬度有正相关,这表明它在保持坚硬度方面发挥了作用.
结论:
- 低温储存通过抑制pectin降解来保持桃子的坚硬性.
- PpCBFs,特别是PpCBF2和PpCBF3,通过直接促进体结合,在pectin降解酶基因 (PpPL2和PPG) 的表达中发挥负面调节作用.
- 激活PpCBF是一种关键机制,通过低温在储存期间保持桃子水果的质量.
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