在丝虫Bombyx mori中进行复杂的,多种酶介导的天然植物颜料合成工程
Kai Chen1,2, Ye Yu1,2, Zhongjie Zhang1,2
1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
概括
经过基因改造的丝虫现在可以通过表达关键合成基因来产生植物性贝他林色素. 这一突破使昆虫转基因中的可见标记物成为可能,并利用丝虫作为有价值化合物的生物反应器.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 昆虫分子生物学 昆虫分子生物学
背景情况:
- 自然植物颜料提供了宝贵的资源,但将它们的合成适应动物是具有挑战性的.
- 贝塔林是Caryophyllales的水溶性颜料,具有复杂的生物合成途径,涉及多种酶.
- 对复杂植物颜料生产的动物进行基因工程仍然是一个未经探索的领域.
研究的目的:
- 在非植物系统中实现植物性贝他林的生物合成,特别是转基因丝虫.
- 探索使用转基因昆虫作为生物反应器来制造有价值的天然化合物的潜力.
- 确定贝他林作为昆虫转基因的可见标记物.
主要方法:
- 在丝虫中,三个关键的贝他林合成基因 (CYP76AD1,DOPA 4,5-二氧化酶,贝他尼丁5-O-葡萄糖转移酶) 的同时表达.
- 利用无处不在的 (IE1) 和丝腺特异性 (纤维素重链) 促进剂来控制基因表达.
- 在血淋巴和从子中恢复中的贝塔林积累的量化.
主要成果:
- 在转基因丝虫中成功生物合成贝他林,在血淋巴中累积高达274μg/ml.
- 转基因幼虫和虫呈现出鲜明的红色,作为可见标记.
- 贝塔林被分泌到子中,从子中产生14.4微克/毫克,表明有效的恢复.
结论:
- 昆虫能够合成复杂的,多酶介导的植物颜料.
- 基因工程丝虫可以作为有效的生物反应器,用于生产植物衍生化合物,如贝他林.
- 贝塔林在昆虫转基因中起到可靠的可见标记的作用,简化了转型个体的识别.
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