相关实验视频

Updated: Jul 19, 2025

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

在寒冷适应过程中,NTRC调节CP12以激活卡尔文-本森循环

Jing Tsong Teh¹, Verena Leitz², Victoria J C Holzer¹

¹Department of Molecular Plant Science, Faculty of Biology, Ludwig-Maximilians-Universität Munich, Planegg 82152, Germany.

Proceedings of the National Academy of Sciences of the United States of America

|August 7, 2023

概括

依赖NADPH的硫素还原酶C (NTRC) 调节叶绿体并调节寒冷耐受性. 它降低了CP12,控制了植物适应寒冷所必需的关键酶综合体.

科学领域:

植物生物学植物生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

叶绿体的氧化还原调节对于植物的适应至关重要.
依赖NADPH的硫素还原酶C (NTRC) 是叶绿体中一个关键的氧化还原调节剂.
寒冷压力显著影响植物生理学和酶活性.

研究的目的:

为了研究NTRC在*Chlamydomonas reinhardtii*的寒冷耐受性中的作用.
阐明NTRC调解寒冷适应的分子机制.
确定参与冷反应的NTRC的交互合作伙伴.

主要方法:

对NTRC活性部位的特定位点突变分析.
共同免疫沉和质谱测量以确定蛋白质相互作用.
在体外生化分析包括尺寸排除色谱.
基于CRISPR的基因编辑,以创建突变菌株.

主要成果:

NTRC以氧化还原依赖的方式调解寒冷耐受性.
在体外,NTRC直接降低了卡尔文-本森循环蛋白12 (CP12).
NTRC控制PRK/CP12/GAPDH复合体的完整性,影响在寒冷中酶的活性.
删除CP12会影响PRK和GAPDH蛋白水平,这表明它有保护作用.

结论:

通过NTRC介导的CP12降低会触发PRK/CP12/GAPDH复合体在寒冷中解离的过程.
CP12在酶复合体的完整性和蛋白质保护中起着双重作用,对寒冷适应至关重要.
这项研究揭示了植物寒冷适应的关键氧化还原依赖机制.

关键词:

CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 CP12 在NTRC中,NTRC是指NTRC.碳代谢碳代谢碳代谢寒冷适应习惯的过程.

更多相关视频

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

相关概念视频

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

The Calvin Cycle

The Calvin Cycle

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...