在酵母中发现动态植物酶综合体,用于识别Kratom类化合物途径
Yinan Wu1, Chang Liu1, Anna Koganitsky1
1Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, 14853, Ithaca, NY, USA.
Angewandte Chemie (International ed. in English)
|August 7, 2023
概括
研究人员开发了一种基于酵母的查方法,以寻找植物酶相互作用,在 kratom 中发现了新的天然产品途径. 这种方法有助于识别药用植物的复杂生物合成路径.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 自然产品的发现自然产品的发现.
背景情况:
- 在药用植物中确定天然产品生物合成途径是复杂且耗时的.
- 转录组调节和酶合调节是确定途径的既定方法.
- 蛋白与蛋白相互作用 (PPI) 为发现途径组织提供了一个互补的方法.
研究的目的:
- 开发和验证一种基于酵母的选方法,用于捕获植物酶PPI.
- 在化物生产植物,kratom中发现新的酶复合物和生物合成途径.
- 整合PPI数据与多组学分析,以加强路径发现.
主要方法:
- 基于酵母的蛋白质与蛋白质相互作用 (PPI) 查.
- 植物样本的多组学分析.
- 已识别的酶的生物化学表征.
主要成果:
- 在 kratom 中发现了四个酶复合体和有组织的生物合成途径.
- 鉴定了六个中链脱酶/减少酶 (MsMDRs) 与狭胺β-D-葡萄糖酶 (MsSGD) 相互作用.
- 验证了四个 MsMDR 的功能,证明了 PPI 查的高选择性.
结论:
- 利用蛋白质-蛋白质相互作用是发现植物天然产品生物合成途径的强大策略.
- 开发的基于酵母的PPI查方法有效地补充了多组学方法.
- 这项研究为探索途径阐明中的翻译后调节开辟了新的途径.
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