扩展生物催化化级联涉及扩散FADH的单聚再生系统2
Nicolai Montua1, Norbert Sewald1
1Organic and Bioorganic Chemistry, Department of Chemistry, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
Chembiochem : a European journal of chemical biology
|August 7, 2023
概括
这项研究引入了一种新型的双功能酶,用于高效的黄素再生,简化了基托衍生物的生产. 这种精简的过程使得可扩展的,一合成有价值的化合物,如二和金氨酸.
科学领域:
- 生物催化剂是一种生物催化剂.
- 合成有机化学 合成有机化学
- 酶工程是什么?酶工程是什么?
背景情况:
- 黄素依赖的化酶提供区域选择性C-H化,但需要复杂的多步骤酶生产.
- 现有的联合交联酶聚合物 (combiCLEAs) 的现有方法涉及多种培养和净化步骤.
- 需要简化,可扩展的生物催化系统来生产化芳香化合物.
研究的目的:
- 开发一种精简的,共同表达的系统,用于生产依赖黄素的类酶及其再生酶.
- 为了使酶能够从单一种植中无载体固定到组合CLEAs中.
- 为了证明这个系统的可扩展性和应用性,用于二和金氨酸衍生物的准备合成.
主要方法:
- 一个双功能的flavin再生酶与flavin依赖的托芬基酶的同时表达.
- 使用单一种植方法开发无载体组合CLEAs.
- 通过在基板生物反应器中进行料批发发酵来评估可扩展性.
- 为随后的反应级联集成一个6 - 三胺特异性二氧化酶.
主要成果:
- 一种能够利用酸盐进行两步催化原蛋白再生的融合蛋白与Trp-halogenases成功地共同表达.
- 无载体组合CLEAs在单一种植中得到了高效的生产,简化了酶生产和固定.
- 该系统的可扩展性高达2.5L,促进了二衍生物的准备合成.
- 为了合成l-4-Cl-kynurenine及其化模拟物,建立了一个单反应级联.
结论:
- 开发的双功能酶和联合表达策略显著简化了依赖黄素的类酶的生产和应用.
- 这种方法使得可扩展和高效的无载体组合CLEAs能够用于复杂化代谢物的预制合成.
- 单级联反应扩大了该系统用于生产有价值的金氨酸衍生物的实用性.
相关概念视频
02:17Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.4K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.4K
02:46Halogenation of Alkenes
15.8K
Halogenation is the addition of chlorine or bromine across the double bond in an alkene to yield a vicinal dihalide. The reaction occurs in the presence of inert and non-nucleophilic solvents, such as methylene chloride, chloroform, or carbon tetrachloride.
Consider the bromination of cyclopentene. Molecular bromine is polarized in the proximity of the π electrons of cyclopentene. An electrophilic bromine atom adds across the double bond, forming a cyclic bromonium ion intermediate.
Consider the bromination of cyclopentene. Molecular bromine is polarized in the proximity of the π electrons of cyclopentene. An electrophilic bromine atom adds across the double bond, forming a cyclic bromonium ion intermediate.
15.8K
01:14Electrophilic 1,2- and 1,4-Addition of X2 to 1,3-Butadiene
2.5K
Electrophilic addition of halogens to alkenes proceeds via a cyclic halonium ion to form a 1,2-dihalide or a vicinal dihalide.
2.5K
02:17Regioselectivity of Electrophilic Additions to Alkenes: Markovnikov's Rule
14.3K
If a set of reactants can yield multiple constitutional isomers, but one of the isomers is obtained as the major product, the reaction is said to be regioselective. In such reactions, bond formation or breaking is favored at one reaction site over others.
The hydrohalogenation of an unsymmetrical alkene can yield two haloalkane products, depending on which vinylic carbon takes up the halogen. However, one product usually predominates, where hydrogen adds to the vinylic carbon bearing the...
The hydrohalogenation of an unsymmetrical alkene can yield two haloalkane products, depending on which vinylic carbon takes up the halogen. However, one product usually predominates, where hydrogen adds to the vinylic carbon bearing the...
14.3K
01:26Radical Substitution: Hydrogenolysis of Alkyl Halides with Tributyltin Hydride
1.8K
Radical substitution reactions can be used to remove functional groups from molecules. The hydrogenolysis of alkyl halides is one such reaction, where the weak Sn–H bond in tributyltin hydride reacts with alkyl halides to form alkanes. Here, the reagent Bu3SnH yields tributyltin halide as a byproduct.
The bonds formed in this reaction are stronger than the bonds broken, making it energetically favorable. The reaction follows a radical chain mechanism similar to radical halogenation...
The bonds formed in this reaction are stronger than the bonds broken, making it energetically favorable. The reaction follows a radical chain mechanism similar to radical halogenation...
1.8K
01:10Radical Chain-Growth Polymerization: Overview
2.5K
Chain-growth or addition polymerization is successive addition reactions of monomers with a polymer chain. In radical chain-growth polymerization, the reaction proceeds via a free-radical intermediate. The free radical is formed from radical initiators, which spontaneously generate free radicals by homolytic fission. Organic peroxides (such as dibenzoyl peroxide, as shown in Figure 1) or azo compounds are popular radical initiators. A low concentration ratio of radical initiator to monomer is...
2.5K