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综合分子网络分析揭示了与不孕症相关的关键基因和转录因子在非阻断性阿佐精子症中的转录因子

Dhananjay B Alagundagi¹, Sudeep D Ghate², Prasannakumar Shetty³

¹Central Research Laboratory, K S Hegde Medical Academy, NITTE (Deemed to be University), Mangaluru 575018, Karnataka, India.

European journal of obstetrics, gynecology, and reproductive biology

|August 7, 2023

概括

这项研究确定了TEX38和FAM71F等关键基因,这些基因在非阻塞性精症 (NOA) 中被上调. 这些基因及其调节器显示出作为男性不孕症的诊断和预后生物标志物的潜力.

科学领域:

生殖生物学生殖生物学
遗传学是一个遗传学.
生物信息学是一种生物信息学.

背景情况:

男性不孕症是一个复杂的问题,非阻塞性精子缺血症 (NOA) 的特征是精子生成失败.
对NOA的潜在分子机制的理解仍然很差.

研究的目的:

确定与男性不孕症相关的关键基因,以作为在精中诊断和预后生物标志物的潜在用途.
阐明NOA所涉及的分子途径和调节因素.

主要方法:

对微阵列数据集 (GSE45885,GSE45887) 的分析,以确定NOA中的差异表达基因 (DEGs).
构建蛋白质-蛋白质相互作用 (PPI) 网络以识别枢纽基因和调控转录因子 (TF) 和蛋白质激酶.
使用独立的微阵列数据集 (GSE190752) 验证枢纽基因表达,并分析丸活检.

主要成果:

鉴定了256个DEG并构建了一个PPI网络,揭示了NOA中调高的枢纽基因 (例如,TEX38,FAM71F,PRR30).
与这些基因相关的生物过程包括精子生成和精子发育.
与对照组相比,NOA受试者的丸活检中验证了枢纽基因的增加表达,其中23个TF和3个蛋白激酶被确定为调节剂.

结论:

在这项研究中发现的关键基因及其相关的转录因子调节了NOA.中的不孕症相关过程.
这些枢纽基因的验证表达表明它们有可能作为NOA的诊断或预后生物标志物.

关键词:

不同表达的基因.综合性分析是一种综合性分析.男人不孕不育的情况非阻塞性阿佐精子症.转录因子转录因子

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