同时去除抗生素并通过生物氧化过程减轻耐药诱导
Chong-Yang Ren1, Qiu-Jin Xu1, Pedro J J Alvarez2
1MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou, 310058, China.
Water research
|August 7, 2023
概括
这项研究表明,Pseudomonas aeruginosa MQ2的生物氧化会降解抗生素,并减少抗生素耐药性基因的传播. 这一过程减轻了突变和水平基因转移,为水处理提供了一种新的方法.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 处理水处理水处理水处理
背景情况:
- 从废水中去除抗生素至关重要.
- 生物治疗可以通过突变和水平基因转移 (HGT) 增加抗生素耐药性基因 (ARG) 传播.
研究的目的:
- 研究生物氧化的潜力,同时降解抗生素和减轻抗生素耐药性.
- 了解生物氧化对HGT和突变的影响背后的机制.
主要方法:
- 使用了一个 (II) 氧化细菌 (MnOB),Pseudomonas aeruginosa MQ2.2.
- 研究了二氧化,生物性氧化物 (BioMnOx) 的形成,以及它们对抗生素降解和ARG转移的影响.
- 进行了转录基因分析以阐明分子机制.
- 建造了一个基于的生物氧化反应器,用于去除四环素.
主要成果:
- Pseudomonas aeruginosa MQ2同时降解了抗生素,并降低了HGT和突变率.
- 生物氧化物 (BioMnOx) 覆盖了细菌细胞,并有效氧化了西普洛素 (93%) 和四环素 (96%).
- 转录组分析揭示了参与Mn(II) 氧化的特定基因,并确定了与氧化应激,HGT和多药物耐药性相关的基因的下调.
- 一个试验反应堆在4小时的液压保留时间内完全消除了环境中的四环素度.
结论:
- 生物氧化是降解废水中的抗生素的一个有希望的策略.
- 这一过程有效地通过减少HGT和突变来缓解抗生素耐药性的传播.
- 这些发现表明了控制水处理系统中抗生素污染和耐药性传播的新方法.
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