模拟聚乳酸 (PLA) 可生物降解的微塑料的胃肠道消化及其与肠道微生物群的相互作用
C Jiménez-Arroyo1, A Tamargo1, N Molinero1
1Institute of Food Science Research, CIAL, CSIC-UAM, C/ Nicolás Cabrera 9, 28049 Madrid, Spain.
The Science of the total environment
|August 7, 2023
概括
聚乳酸 (PLA) 微塑料可能会改变人类肠道微生物的新陈代谢,并显示生物降解的迹象. 进一步的研究对于了解这些生物塑料在人类胃肠道中的健康影响至关重要.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物材料科学 生物材料科学
背景情况:
- 微塑料 (MP) 在食物和人类中的积累需要进行健康影响研究.
- 聚乳酸 (PLA) 是一种广泛使用的生物塑料,具有"普遍认可为安全" (GRAS) 状态.
- 动物研究表明,PLA MPs可以改变肠道微生物群,但人类肠道后果仍未研究.
研究的目的:
- 模拟每天摄入的PLA MP并评估对人类结肠微生物群组成和代谢功能的影响.
- 为了研究人类肠道微生物群对PLA MPs的潜在生物转化.
- 评估在胃肠道过境期间PLA MPs的形态变化和生物降解.
主要方法:
- 利用INFOGEST方法和胃肠道simgi®模型进行模拟消化.
- 使用16S rRNA基因测序分析了结肠微生物组合.
- 量化乳酸和短链脂肪酸 (SCFA) 的生产.
- 使用拉曼光谱和场辐射扫描电子显微镜 (FESEM) 描述了PLA MP形态和表面变化.
主要成果:
- PLA MPs并没有显著改变整体微生物社区的恒温,但显示了增加Bifidobacterium与毫米颗粒的趋势.
- 检测到的功能转变表明了微生物代谢的改变和结肠微生物群的潜在PLA生物转化.
- 在消化后观察到PLA MPs的形态变化,有机物粘附,生物膜形成和表面生物降解.
结论:
- 摄入的PLA MPs可能会受到人类结肠微生物群的生物转化和表面生物降解.
- 肠道微生物群中的功能性转变表明,由于暴露于PLA MP而导致的潜在代谢变化.
- 需要进一步研究生物塑料在胃肠道中对人类健康的影响和生物降解.
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