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针对粉样蛋白前体蛋白加工机械的miRNAs在细胞外囊泡和来自口腔状细胞的颗粒中产生粉样蛋白前体癌

Fernando Galvão¹, Michele Patricia Rode², Paloma Santos de Campos³

¹Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

|August 7, 2023

概括

针对细胞外囊和颗粒 (EVP) 中的粉样前体蛋白 (APP) 处理的微RNA (miRNA) 在口腔状细胞癌 (OSCC) 中发生变化. 这些发现表明在OSCC发育和作为瘤抑制剂的潜在作用.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

口腔状细胞癌 (OSCC) 与microRNAs (miRNAs),细胞外囊泡和颗粒 (EVPs) 和粉样前体蛋白 (APP) 处理的改变有关.
在EVP中调查针对APP处理途径的miRNAs对于理解OSCC病原性至关重要.

研究的目的:

为了评估来自OSCC细胞系的EVP中向APP或ADAM10的miRNAs.
将攻击性和非攻击性OSCC细胞系中的miRNA配置文件与正常的角质细胞进行比较.

主要方法:

使用了两个OSCC细胞系 (SCC09,CAL27) 和一个角质细胞系 (HaCaT).
隔离了EVP并通过纳米追踪分析分析了它们的尺寸和度.
针对APP或ADAM10的特定miRNAs (miR-20a-5p,miR-103a-3p,miR-424-5p,miR-92b-3p,miR-31-5p,miR-93-5p) 的量化水平.

主要成果:

在细胞系之间没有观察到EVP大小分布或度的显著差异.
与HaCaT相比,OSCC细胞系的EVP显示上调的miR-20a-5p,miR-92b-3p和miR-93-5p.
发现miR-31-5p在来自CAL27细胞的EVP中被降低.

结论:

确定了来自OSCC细胞系的EVP内针对APP处理机制的miRNAs的变化,其攻击性各不相同.
这些变化可能导致OSCC组织中异常miRNA表达.
这项研究表明,这些miRNAs在OSCC中作为瘤抑制剂的潜在作用.

关键词:

癌症癌症癌症癌症癌症外基因组是外基因组的组成部分.这就是miRNAs.微小的微小的微小的微小的微小非粉原性通路的非粉原性通路

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