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通过对直立显微镜的灵活,低成本的调整,高灵敏度的内在光学信号成像

Brenda Vasquez¹, Baruc Campos¹, Ashley Cao¹

¹Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095.

|August 7, 2023

概括

这项研究提出了一种负担得起的方法来升级标准显微镜,用于内在光学信号成像 (IOSI),从而实现详细的脑活动映射. 这种可适应的系统为神经科学研究提供了可重复的结果,即使预算有限.

科学领域:

神经科学是一个神经科学.
光学成像技术的成像
显微镜的使用方法

背景情况:

内在光信号成像 (IOSI) 对于绘制大脑活动至关重要,但缺乏负担得起的商业系统.
研究人员经常构建定制的IOSI设置,导致变化和高成本.
现有的IOSI系统无法广泛使用,这阻碍了神经科学实验室的更广泛采用.

研究的目的:

为标准直立显微镜提供一个具有成本效益的适应,以执行高灵敏度的IOSI.
通过使用易于获得的组件,使大脑皮层中神经元活动的宏观映射成为可能.
为IOSI系统控制和数据分析提供开源工具.

主要方法:

适应标准的直立显微镜,使用廉价的商业部件用于照明,光学和检测.
利用适应的系统在小鼠的体感和视觉皮层绘制传感唤起的信号.
开发开源软件,用于控制照明和分析IOSI数据.

主要成果:

成功生成了皮质活动的高灵敏度IOSI地图,包括单胡子桶地图.
在不同动物中证明了IOSI地图的高可重现性.
验证了IOSI地图的使用,用于研究体感皮层中的可塑性机制.

结论:

提出的IOSI适应为神经科学实验室提供了一个预算友好的解决方案.
这种方法使获得先进的大脑活动映射技术的访问变得民主化.
开源资源促进了IOSI在各种研究环境中的实施和使用.

关键词:

皮质的塑性皮质的塑性在活体中成像成像内在信号光学成像内部信号光学成像人体感官皮层 (somatosensory cortex) 是一个感官皮层.

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