一种植物光系统II超级复合体的冷EM结构,其中包含采光蛋白Lhcb8和α-托科菲罗尔
Monika Opatíková1, Dmitry A Semchonok2, David Kopečný3
1Department of Biophysics, Faculty of Science, Palacký University, Olomouc, Czech Republic.
Nature plants
|August 7, 2023
概括
研究人员揭示了非开花植物松树中光系统II (PSII) 超级复杂的结构. 这种结构包括一种新的α-托科菲罗尔/α-托科菲罗尔农分子,在光合作用过程中可能会感知过多的光线.
科学领域:
- 植物分子生物学 植物分子生物学
- 光合作用研究研究光合作用.
- 结构生物学是结构生物学.
背景情况:
- 氧气光合作用依赖于光系统II (PSII) 的超级复合体.
- PSII超级综合体集成光采集综合体 (LHCII).
- 以前的结构数据主要来自开花植物和藻类,缺乏非开花植物的代表性.
研究的目的:
- 通过冷电子显微镜测定 PSII 超级复合物的结构.
- 调查非开花植物的结构差异,并确定非开花植物的新组成部分.
主要方法:
- 低温电子显微镜 (cryo-EM) 在2.8 Å分辨率.
- 结构分析和与现有的PSII超复杂数据进行比较.
主要成果:
- 获得了松树PSII超级综合体的高分辨率结构.
- 树PSII超级复合体包含一个额外的Ycf12子单位和Lhcb8而不是Lhcb4.
- 在LHCII-CP43边界确定了一种新型α-托科法罗 (α-Toc) /α-托科法罗 (α-TQ) 分子.
结论:
- 已识别的α-Toc/α-TQ分子处于战略位置,可以作为过度光应激的传感器.
- 松树PSII的结构变化表明非开花植物的适应.
- 这些发现为PSII超级综合体的监管和结构功能关系提供了新的见解.
相关概念视频
Photosystem II
71.1K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
71.1K
Photosystems
4.9K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
4.9K
The Antenna Complex
6.0K
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency...
6.0K
Photosystem I
63.0K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
63.0K
The Photochemical Reaction Center
4.2K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.2K
Electron Transport Chain: Complex III and IV
7.6K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.6K


