分离和识别来自葡萄的自发发酵的本土酵母
Liliana Mabel Gerard1, María Belén Corrado2, Cristina Verónica Davies2
1Laboratorio de Microbiología y Biotecnología de Alimentos, Facultad de Ciencias de la Alimentación, Universidad Nacional de Entre Ríos, Monseñor Tavella 1450, 3200, Concordia, Entre Ríos, Argentina. liliana.gerard@uner.edu.ar.
Archives of microbiology
|August 7, 2023
概括
这项研究在阿根廷的自发葡萄酒发酵过程中发现了包括Pichia kudriavzevii在内的本地酵母. 在多个年份的葡萄酒中,Pichia kudriavzevii一直存在,这突出了它在区域葡萄酒特征中的作用.
科学领域:
- * 葡萄酒学和微生物学:专注于本地酵母的特征,用于区域葡萄酒生产.
背景情况:
- * 越来越多的人对利用本土酵母来制造独特的葡萄酒的兴趣.
- *自发发酵依赖于自然存在的酵母种群.
研究的目的:
- * 在Tannat和Marselan葡萄酒中研究Saccharomyces和非Saccharomyces酵母.
- *分析来自阿根廷康科迪亚的酵母种群,跨越2019年至2021年的年份.
- * 酵母因其对区域葡萄酒特征的潜在贡献而具有特征.
主要方法:
- * 对发酵参数的监测:可溶性固体总量,酸度,pH,乙醇和总碳.
- *使用殖民地形态学,5.8S-ITS-RFLP和26S rDNA D1/D2基因测序来隔离和识别酵母.
- * 在自发发酵过程中对酵母多样性的分析.
主要成果:
- *确定了210个酵母菌群,包括Pichia kudriavzevii,Saccharomyces cerevisiae和汉斯尼亚斯波拉物种.
- *Pichia kudriavzevii是从所有三个年货中分离出来的.
- * 在本地葡萄酒的自发发酵中观察到特定的酵母形状.
结论:
- *本地酵母种群,特别是Pichia kudriavzevii,在康科迪亚的自发葡萄酒发酵中发挥着重要作用.
- *这些发现支持使用土著酵母开发具有独特区域特征的葡萄酒的潜力.
- *进一步的研究可以探索已识别的酵母菌株的特定酒特性.
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