相关实验视频
Updated: Jul 19, 2025
10:36
Probiotic Studies in Neonatal Mice Using Gavage
Published on: January 27, 2019
19.5K
在早产婴儿的肠道微生物群中,基线阿兹罗密辛耐药性
David J Gallacher1,2, Lei Zhang2, Ali F Aboklaish2
1Neonatal Unit, University Hospital of Wales, Cardiff, UK.
Pediatric research
|August 7, 2023
概括
宏类耐药性在早产婴儿中很常见.
科学领域:
- 微生物学 微生物学
- 新生儿医学 新生儿医学
- 遗传学 是一个遗传学.
背景情况:
- 麦克罗利德,如阿齐思罗米,越来越多地用于早产婴儿的Ureaplasma感染.
- 在早产婴儿的肠道微生物群中,对宏类耐药基因的基线携带并未得到充分理解.
研究的目的:
- 为了在早产婴儿中识别抗阿兹胺素的细菌.
- 为了确定这些细菌中宏类耐药基因的发生率.
主要方法:
- 从早产婴儿 (孕期≤32周) 的连续便样本中分离出耐亚兹罗米辛的细菌.
- 在有氧和无氧条件下培养,有或没有阿齐思罗米.
- 定量PCR检测六种常见的宏类耐药基因 (erm(A), erm(B), erm(C), erm(F), mef(A/E), msr(A)) 在耐药单独体中.
主要成果:
- 从37名婴儿的89个便样本中的93.3%观察到细菌生长.
- 在280个抗阿齐菌素的隔离物中,Staphylococcus (75%) 和Enterococcus (15%) 种类占主导地位.
- 在91%的耐药分离物中存在宏类耐药基因,其中ermC (46%) 和msrA (40%) 是最常见的. 在18%的隔离物中发现了多个基因.
结论:
- 在早产婴儿的肠道微生物群中,宏类耐药性在生命早期很普遍.
- 耐药性很可能是从母体微生物群暴露中获得的.
- 如果在早产婴儿护理中使用化物成为常规,进一步评估化物耐药性调节至关重要.
相关概念视频
01:23Anatomy of the Intestines
72.4K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
72.4K
01:22Development of Immunocompetence
344
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
344