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Updated: Oct 23, 2026

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

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一个实地可部署的实时循环介导的同热放大,准5个nrdB基因拷贝,用于检测类植物中的"Candidatus Liberibacter asiaticus"

Tirumalareddy Danda¹, Jong-Won Park¹, Kimberly L Timmons¹

¹Texas A&M University-Kingsville Citrus Center, 312 N. International Blvd., Weslaco, TX 78599, USA.

The plant pathology journal

|August 8, 2023

概括

一种新的实时循环介导同热放大 (rt-LAMP) 试验,使用原始DNA提取物在果中快速检测黄龙 (HLB). 这种现场部署的方法为HLB提供了可靠的查,补充了传统的qPCR诊断.

科学领域:

植物病理学植物病理学
分子诊断学分子诊断学
细菌学细菌学是一门学科.

背景情况:

黄龙 (HLB) 是一种在全球范围内毁灭性的类病,由亚洲候选菌 (Candidatus Liberibacter asiaticus,CLas) 引起.
目前的诊断方法,如定量实时聚合酶连锁反应 (qPCR) 是敏感的,但实验室依赖和耗时.
需要快速,可在现场部署的诊断工具,以有效管理HLB.

研究的目的:

开发和验证一个实时循环介导的同热放大 (rt-LAMP) 试验,用于快速检测CLas.
为了评估rt-LAMP测试在各种类样本和亚洲类植物 (ACP) 中的性能.
评估使用原始DNA提取物用于基于现场的HLB查的可行性.

主要方法:

开发一种rt-LAMP试验,针对CLas五副本的nrdB基因.
用各种植物组织 (叶子,树皮,根) 和ACP.测试试剂.
将rt-LAMP结果与用于CLas检测的基于实验室的qPCR进行比较.

主要成果:

rt-LAMP试验成功检测到CLas在~2.6Log10副本的水平.
与qPCR相比,树叶,树皮和ACP中的CLas检测率超过了96%.
纤维根的检测率低于qPCR,这是由于一些样本中的CLas标位较低.
测试表明,使用原始叶子DNA提取物证明了有效性.

结论:

开发的rt-LAMP检测方法为现场HLB查提供了快速可靠的方法.
这种测试可以使用原始DNA提取物,简化样品的准备.
虽然在某些情况下比qPCR稍微不太敏感,但rt-LAMP为快速检测和管理HLB决策提供了有价值的工具.

关键词:

在Candidatus Liberibacter asiaticus的研究中.类的类植物.黄龙平是什么意思黄龙平是什么意思循环介导的同热放大方式.这里是nrdBB.

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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