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在压力下的小鼠中,GGA1参与精子生成

Haoyun Jiao¹, Yinghong Chen^2,3,4, Tingting Han¹

¹The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology of National Health Commission, Beijing, P.R. China.

|August 8, 2023

概括

戈尔吉局部化,含有玛适配素的耳朵,ARF结合蛋白1 (GGA1) 保护男性生育能力免受双A (BPA) 暴露. 缺乏GGA1的小鼠在接触BPA时变得不育,表现出受损的精子生成.

科学领域:

生殖生物学生殖生物学
环境毒理学环境毒理学
分子遗传学分子遗传学

背景情况:

在全球范围内,男性不孕症影响了大约50%的人类生殖病例.
环境中有毒物质,如双A (BPA),是男性不孕症的重要贡献者.
BPA 破坏了对精子生成至关重要的戈尔吉器官功能,GGA1 在哺乳动物生殖中的作用尚不清楚.

研究的目的:

研究GGA1在哺乳动物精子生成中的作用.
为了确定GGA1是否保护男性生殖功能免受BPA诱导的毒性.

主要方法:

使用CRISPR/Cas9.9生成了Gga1的淘汰赛小鼠.
每天给BPA (40μg/kg) 的腹腔内注射,持续2周,给淘汰和野生型小鼠.
分析丸组织学和免疫光染色以评估生殖参数.

主要成果:

淘汰赛中的Gga1小鼠表现出正常的生育能力和精子生成,没有暴露于BPA.
在Gga1淘汰赛小鼠中,BPA暴露导致不孕,精子数量减少,运动性和形态正常.
组织学分析显示,BPA暴露的Gga1无菌小鼠的种子表皮有失组织,并增加了生殖细胞亡.

结论:

在环境压力下,Gga1对于维持男性生育能力至关重要.
GGA1在精子生成中起着保护作用,防止BPA引起的损伤.
向GGA1可能为由环境毒素引起的男性不孕症提供治疗策略.

关键词:

BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA Gga1ga1 在线观看精子计数数的精子数量精子形态精子形态精子发生是精子生成.

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