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转录组和甲基组测序揭示了长非编码RNA基因表达的改变及其异常DNA甲基化在马类肉类体中

Ewelina Semik-Gurgul¹, Artur Gurgul², Tomasz Szmatoła^3,2

¹Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 St., 32-083, Krakow, Balice, Poland. ewelina.semik@izoo.krakow.pl.

Functional & integrative genomics

|August 8, 2023

概括

这项研究揭示了长非编码RNAs (lncRNAs) 在常见的马皮肤瘤 - - 马类瘤中的作用. 研究人员确定了许多差异表达的lncRNA及其向基因,为这种牛乳头瘤病毒依赖性瘤提供了新的见解.

科学领域:

兽医医学兽医医学兽医医学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

长非编码RNAs (lncRNAs) 是基因转录的关键调节者.
lncRNAs以前没有在马类肉骨瘤中进行表征,这是一种马类显著的皮肤瘤.
了解lncRNA表达对于阐明马类肉类的病原性至关重要.

研究的目的:

为了研究马类肉骨动物的lncRNA表达特征.
为了确定参与这种牛乳头瘤病毒依赖性瘤的发病的新型 lncRNA.
探索马皮组织中 lncRNAs 的调节功能.

主要方法:

重新分析来自12个马类肉骨样本和对照组的RNA测序 (RNA-seq) 数据.
差异表达 (DE) lncRNAs及其相关差异表达基因 (DEGs) 的识别.
整合甲基化数据以评估异常甲基化对DElncRNAs的影响.

主要成果:

确定了3396个DE lncRNA和128个DElncRNA-DEG对.
预测DE lncRNAs的目标基因在与细胞外矩阵分解和癌症相关的途径中得到丰富.
12 DElncRNAs被发现可能受到异常甲基化的影响.

结论:

这项研究提供了关于 lncRNAs 在马类沙科菌病原发生的作用的新见解.
已识别的 lncRNA 和路径为未来的研究和治疗策略提供了潜在的目标.
lncRNAs与马类牛乳头瘤病毒依赖的瘤过程有关.

关键词:

DElncRNAs 的使用情况.通过DNA甲基化.不同表达的基因.马类的肉类动物.长的非编码RNA是什么?转录组转录组就是一个转录组.瘤是一个瘤.

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