基于废水的SARS-CoV-2生物标志物的流行病学:评估德国南部小型和大型社区的规范化方法
Alexander Mitranescu1, Anna Uchaikina1, Anna-Sonia Kau1
1Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748Garching, Germany.
概括
基于废水的流行病学 (WBE) 有效地追踪SARS-CoV-2. 使用便指标的组合来规范数据,如CrAssphage和胡轻度斑点病毒,提高了准确性,但最佳方法因地点而异.
科学领域:
- 环境科学 环境科学
- 流行病学 流行病学
- 公共卫生 公共卫生
背景情况:
- 基于废水的流行病学 (WBE) 对于监测COVID-19等传染病至关重要.
- 废水中的SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 流行数据需要正常化,以考虑稀释和提高准确性.
- 在德国南部的研究期间 (2021年8月至12月) 占主导地位的Delta变种.
研究的目的:
- 评估SARS-CoV-2 WBE.各种规范化参数的有效性.
- 为了评估流动的性能,替代病毒 (CrAssphage,胡温和斑点病毒) 和水化学数据进行正常化.
- 确定影响正常化参数性能的特定场所因素.
主要方法:
- 来自德国南部大小社区的废水样本的分析.
- 使用影响流量,降水数据,CrAssphage和胡轻度斑点病毒来调查正常化.
- 对不同采样地点和时间变化的正常化性能进行比较.
主要成果:
- 影响流量正常化显示对降水事件的敏感性.
- 取代病毒正常化性能在采样地点之间有显著差异.
- 一种混合便指标,结合了CrAssphage和胡轻度斑点病毒,显示出最好的整体正常化性能.
- 对规范化参数的时间和空间变化的分析有助于解释性能差异.
结论:
- 规范化参数 (替代病毒,流量,水化学数据) 的有效性高度与特定地点有关.
- 对于特定的污水系统,建议对规范化参数进行单独测试,以优化WBE的准确性.
- 特定地点的规范化策略对于使用废水进行可靠的人口级疾病监测至关重要.
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