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AIE基于纳米酶的长时间持久化学发光和光用于致病细菌的POCT

Haotian Wu¹, Yuan Fang¹, Li Tian²

¹State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.

|August 8, 2023

概括

一种新型聚合诱导排放分子 (AIEzyme) 能够使敏感的,持久的化学发光用于致病性细菌的临床检测 (POCT). 这种没有H2O2的智能手机分析平台,可以在没有外部激发的情况下提供准确的检测.

科学领域:

分析化学分析化学
生物医学工程生物医学工程
材料科学材料科学材料科学

背景情况:

病原细菌对全球健康构成重大风险,需要快速准确的检测方法.
照顾点检测 (POCT) 对于早期发现和治疗细菌感染至关重要.
现有的检测方法往往需要复杂的程序,外部激发源,或缺乏灵敏度.

研究的目的:

开发一种基于化学发光 (CL) 的新型比度传感平台,用于对病原细菌敏感的POCT.
设计和合成一种新的聚合诱导排放 (AIE) 分子,具有类似氧化酶的特性 (AIEzyme).
创建一个便携式,用户友好的生物传感器,不含过氧化 (H2O2) 和外部刺激.

主要方法:

合成了一种新的AIE分子 (AIEzyme),表现出类似氧化酶的活性.
使用醇 (LUM) 和AIEzyme构建一个长时间持久的CL系统.
基于CL共振能量转移 (CLRET) 的比度生物传感器的开发.
自制持有器和智能手机的集成,用于便携式定量分析.

主要成果:

该AIE酶触发了长时间持久的CL明醇超过2小时,归因于反应性氧物种的产生.
分量生物传感器在真实样本中表现出对病原细菌的敏感和定量检测,恢复良好.
开发的平台在没有H2O2或外部激发的情况下运行,简化操作并减少干扰.
长时间持续发光和比度策略的结合提高了检测准确度.

结论:

一个基于AIEzyme的新型CL传感平台为致病细菌的POCT提供了灵敏,准确和便携式的方法.
无H2O2和无激发的设计简化了操作并扩大了适用性.
这个平台为需要点分析提供了一个有前途的方法,包括病原体检测和生物成像.

关键词:

光后的光泽效应 (afterglow luminescence) 是一种光源.聚合诱导的排放 (AIE) 纳米酶细菌检测细菌检测检测长时间持久的化学发光光.医疗点检测 (POCT) 是指在医疗中心进行的检测.

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