与R环相关的G四复合体促进了CTCF结合.
Phillip Wulfridge1, Qingqing Yan1, Nathaniel Rell1
1Gene expression and Regulation program, The Wistar Institute, Philadelphia, PA 19104, USA; Epigenetics Institute, University of Pennsylvania, Philadelphia, PA 19104, USA.
Molecular cell
|August 8, 2023
概括
R-循环和G-四重复 (G4) 结构促进CTCF与DNA结合,影响基因组结构和基因表达. 这一发现揭示了染色质结构如何调节细胞中CTCF占用率.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- CTCF (CCCTC-结合因子) 是基因组架构和基因表达的关键调节者.
- CTCF结合受到DNA序列动机和修改的影响,但并非所有潜在位点都被占用.
- 染色质结构在调节CTCF占用中的作用仍然不完全理解.
研究的目的:
- 调查R环和G四重复 (G4) 结构在调节CTCF结合中的作用.
- 确定染色体结构是否可以影响特定基因组位点的CTCF占用.
主要方法:
- 在小鼠胚胎干细胞中对R循环,G4s和CTCF的同定位研究.
- 在体外试验测试以评估在R环和G4s的存在下CTCF与DNA基因的结合.
- 在体内实验涉及R循环减弱和G4动机删除/稳定.
主要成果:
- 在许多基因组区域,R环和G4s与CTCF共定位.
- 在实验室中,R环和G4s促进CTCF与其DNA基因结合.
- 在体内,R环衰减会降低CTCF结合.
- 删除G4动机减少了CTCF结合,改变了基因表达.
- G4稳定增加了CTCF的占用率,并影响了染色体组织.
结论:
- 通过促进相关G4结构的形成,R环促进CTCF结合.
- 通过CTCF,G4结构在加强长距离基因组相互作用方面发挥着关键作用.
- 像R环和G4s这样的染色体结构元素是CTCF占用和功能的关键调节者.
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