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自组装的SARS-CoV-2尖峰-HBsAg纳米颗粒通过遗传传递引起强大而持久的中和抗体反应

Cuiping Liu¹, Lingshu Wang¹, Jonah S Merriam¹

¹Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, 20892, USA.

|August 8, 2023

概括

结合SARS-CoV-2尖峰和HBsAg蛋白质的新型自组装纳米粒子疫苗显示了对小鼠COVID-19菌株的增强和持久的中和抗体反应.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
病毒学病毒学

背景情况:

目前的COVID-19疫苗需要改进,以提高有效性和耐用性.
SARS-CoV-2 尖端蛋白质是中和抗体的关键目标.
乙型肝炎表面抗原 (HBsAg) 可以增强免疫反应.

研究的目的:

开发和评估针对COVID-19的新型自组装纳米粒子疫苗.
评估SARS-CoV-2尖峰-HBsAg纳米颗粒的免疫性和耐用性.
为了比较纳米粒子疫苗对可溶性和非纳米粒子尖端蛋白的疗效.

主要方法:

自组装的SARS-CoV-2尖峰-HBsAg纳米粒子 (S6P-HBsAgs) 的构建.
用编码为S6P-HBsAgs的DNA免疫小鼠,有或没有先前接种HBV疫苗.
对各种SARS-CoV-2菌株的中和抗体 (nAb) 反应的测量.

主要成果:

证实S6P-HBsAgs与多种SARS-CoV-2单克隆抗体结合.
与对照组相比,用S6P-HBsAgs的DNA免疫引发了明显更强大和更持久的nAb反应.
nAb反应持续时间较长,并且可能通过HBsAg预暴露而增强.

结论:

自组装的S6P-HBsAg纳米粒子比非纳米粒子形式产生更优越和更持久的nAb反应.
这些纳米粒子代表了COVID-19下一代遗传疫苗候选人的有希望.
预暴露HBsAg可能会提高SARS-CoV-2疫苗的疗效.

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