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在骨髓瘤中,CircUSP48通过miR-365调节CYR61来促进恶性行为
Shunguang Chen1, Yan Xu2, Bo Yang3
1Department of Orthopedics, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, 434000, China. 2021710969@yangtzeu.edu.cn.
Functional & integrative genomics
|August 8, 2023
概括
像circUSP48这样的循环RNAs (circRNAs) 通过通过海绵miR-365.5增加CYR61表达来促进骨髓瘤 (OS) 的进展. 准circUSP48可能为OS治疗提供一种新的治疗策略.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 生物化学 生化学
背景情况:
- 循环RNAs (circRNAs) 在癌症发展中至关重要,但hsa_circ_0000028 (circUSP48) 在骨髓瘤 (OS) 中的作用尚未完全理解.
- 骨髓瘤 (OS) 是一种具有显著发病率和死亡率的原发性骨恶性瘤,需要新的治疗点.
研究的目的:
- 研究 circUSP48 在骨髓瘤 (OS) 进展中的功能机制.
- 阐明OS中涉及circUSP48,miR-365和CYR61的调节途径.
主要方法:
- 定量实时PCR (qRT-PCR) 和西式涂抹测量基因和蛋白质表达.
- 细胞增殖试验 (CCK-8,EDU,殖民地形成) 和迁移/入侵试验 (Transwell,scratch).
- 双露西法酶报告器,RNA免疫沉 (RIP) 和RNA拉下测试以确认分子相互作用;活体异种移植模型用于功能评估.
主要成果:
- 在OS组织和细胞中观察到高circUSP48和CYR61表达,低miR-365.
- circUSP48 knockdown 抑制了OS细胞的增殖,迁移和入侵,在体内减少了瘤的生长.
- 循环USP48直接与miR-365相互作用,并通过海绵miR-365对CYR61进行上调;miR-365抑制逆转了circUSP48的淘汰效应.
结论:
- circUSP48通过miR-365海绵机制通过上调CYR61表达来促进骨髓瘤 (OS) 恶性瘤.
- circUSP48代表了骨髓瘤 (OS) 的潜在治疗标.
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