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一个量化器TM三元化HRM查试验,用于准确预测单一来源与混合生物样本的准确预测

Dayanara Torres¹, Chastyn Smith^2,3, Andrea L Williams¹

¹Department of Forensic Science, Virginia Commonwealth University, 1015 Floyd Avenue, Richmond, VA, 23284, USA.

International journal of legal medicine

|August 8, 2023

概括

一种新的法医DNA测定方法集成了QuantifilerTM Trio和高分辨率融 (HRM) 分析,以预测样本类型. 这种方法可以准确地将单一来源的DNA与混合物区分开来,有助于法医案例工作.

科学领域:

法医科学法医科学法医科学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

目前的法医DNA工作流程缺乏预分析能力,以确定样本贡献者的状态 (单一来源与混合).
这种局限性使得对具有挑战性的样本 (如DNA混合物和低模板DNA) 的分析变得复杂.
提前识别样本类型可以实现优化下游处理.

研究的目的:

为了更广泛的适用性,重新设计一个集成的Quantiplex®-HRM试验,变成一个QuantifilerTM Trio-HRM试验.
评估重新设计的试验在量化DNA和预测贡献者状态方面的性能.
评估综合测试与标准法医DNA工作流程的兼容性.

主要方法:

检测的重新设计,包括一种新的DNA结合染料和增加的反应体积.
为所有目标建立新的数据分析和标准曲线指标.
量化和定性值与标准QuantifilerTM Trio化学的比较.
使用综合试验数据生成短串联重复 (STR) 档案.

主要成果:

集成的QuantifilerTM Trio-HRM测定结果与标准QuantifilerTM Trio化学相比,具有可比的量化和定性价值.
产生的STR概况是完整的,与标准方法的概况一致.
试验准确地预测了79.2%的单一来源与混合样本.

结论:

集成的QuantifilerTM Trio-HRM测定显示了改善法医DNA工作流程的巨大潜力.
早期预测样本贡献者状态可以减轻与DNA混合物和低模板DNA相关的挑战.
建议进一步验证和扩展培训套件,以便在法医实验室实际实施.

关键词:

高分辨率的融化曲线 (HRM)混合物检测检测器混合物检测器昆菲勒TM三人组装套件支持矢量机 (SVM) 建模模型 qPCRR 是一个很好的方法.

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