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在卜 (Raphanus sativus L.) 中通过基因组再测序和转录基因组测序开发SNP和InDel标记物

Yadong Li^1,2, Xiaobo Luo³, Xiao Peng^1,2

¹College of Agriculture, Guizhou University, Guiyang, 550003, China.

|August 8, 2023

概括

这项研究确定了卜中数以百万计的单核酸多态 (SNP) 和插入删除 (InDels),提供了有价值的遗传标记. 这些标记物,特别是花开时间基因中的这些标记物,为卜螺栓和花开机制提供了洞察力.

科学领域:

植物基因组学植物基因组学
分子遗传学分子遗传学
农作物科学农作物科学

背景情况:

单核酸多态 (SNPs) 和插入/删除 (InDels) 是植物中丰富的遗传变异.
开发SNP和InDel标记物对于卜 (Raphanus sativus L.) 的遗传和基因组研究至关重要.

研究的目的:

在卜中识别和描述大量的SNP和InDels.
开发用于基因研究的新型InDel标记物.
为了研究卜的开花时间的遗传基础.

主要方法:

两种卜品种 ("YZH"和"XHT") 的基因组重新测序.
转录组测序用于SNP和InDel检测.
聚合酶连锁反应 (PCR) 验证InDel标记物.
种群结构和遗传学分析.
RNA-Seq分析以确定差异表达基因 (DEG).

主要成果:

通过基因组复序确定了366,679个SNP和97,973个InDels.
通过转录组测序检测到53,343个SNP和4,257个InDels.
开发并验证了85个基因组和15个转录基因组InDels.
100个多态的InDel标记在200个卜生殖质中产生了207个等位基因.
确定了11003个DEG,包括145个与开花时间相关的基因.
在与开花时间相关的基因中发现了150个转录基因SNP和9个InDels.

结论:

已识别的SNP和InDels代表了卜遗传和基因组研究的宝贵资源.
花开时间相关基因中的SNP和InDels为卜的螺栓和开花的分子机制提供了洞察力.

关键词:

开花的开花时间遗传多样性遗传多样性在InDel,我们可以看到.人口结构人口结构卜卜卜是什么意思卜卜卜是什么意思转录组转录组就是一个转录组.

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