CryoGrid-PIXUL-RNA:用于组织转录分析的高吞吐量RNA分离平台
Scott A Schactler1,2, Stephen J Scheuerman1,2, Andrea Lius1,2
1UW Medicine South Lake Union, University of Washington, Seattle, WA, 98109, USA.
BMC genomics
|August 8, 2023
概括
一个新的CryoGrid-PIXUL系统通过实现高效的冷存储和采样,简化了组织分析. 这种高通量工作流简化了从组织中分离RNA,为疾病研究提供了具有成本效益的解决方案.
科学领域:
- 生物技术是生物技术.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 高通量多组学需要高效的组织分析工作流程.
- 目前的生物标本存储和采样方法效率低下,造成瓶.
- 皮克苏尔 (PIXUL) 超声波器可从细胞和组织碎片中提取分析物.
研究的目的:
- 开发一个易于使用的系统,用于编目,冷和采样组织碎片.
- 创建一个精简的,高通量RNA分离协议用于组织分析.
- 将一种新的RNA分离方法与现有的组织加工技术相结合.
主要方法:
- 开发用于组织冷存储和采样的CryoGrid系统.
- 使用蛋白酶K (PK) 缓冲器创建基于微板的,无TRIzol和无列的RNA分离协议.
- 将 CryoGrid 系统与 PIXUL 超声波器以及各种 RNA 隔离方法 (PK 缓冲器,TRIzol,PureLink 列) 集成.
主要成果:
- 结合PK缓冲器,TRIzol或PureLink列的CryoGrid-PIXUL系统,产生了可比的RNA转录档案.
- 在毒模型中,分析了从冷的小鼠器官 (大脑,心脏,脏,肝脏) 中的转录档案.
- 证明成功的基因特异性qPCR和全基因组转录分析.
结论:
- 具有96孔微板PK缓冲器方法的CryoGrid-PIXUL系统提供了一个廉价,用户友好的,高吞吐量的工作流.
- 这种综合系统可以有效地研究组织中的转录反应.
- 促进各种疾病的研究,包括健康,疾病和治疗干预措施.
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