与头部到干部发育过渡有关的监管变化.
Patrícia Duarte1, Rion Brattig Correia1, Ana Nóvoa1
1Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156, Oeiras, Portugal.
BMC biology
|August 8, 2023
概括
这项研究揭示了脊椎动物体发育过程中的关键调节网络转移. 基因调节的变化和染色质可访问性控制轴延伸和祖先分化,确保稳健的胚胎发育.
科学领域:
- 发展生物学 发展生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 脊椎动物的胚胎发育包括前部组织的形成,随后是后部轴的延伸,用于干部和尾部的发育.
- 主要的调节基因网络变化与从头部到干部形成的过渡有关.
- 原始的线条和尾茎是驱动顺序轴延伸的关键结构.
研究的目的:
- 研究在脊椎动物胚胎发育过程中管理过渡到树干形成的调节变化.
- 为了确定基因调节网络的变化和轴延伸期间的染色质可访问性.
- 了解Wnt信号和染色质修饰对原生细胞命运的功能影响.
主要方法:
- 从胚胎日7.5和8.5.5的小鼠胚胎中生成差异相互作用网络和染色质可访问性概况.
- 分析细胞过程,信号通路和代谢功能的变化.
- 功能性探索 Wnt 信号传递,包括 Wnt 棕烯酸化,并评估调控元素的染色质可访问性.
主要成果:
- 观察到细胞过程的显著变化,包括Wnt信号传递,无处不在,离子动力学和脂质代谢,在切换到干形成期间.
- 确定了Wnt棕烯酸化相关性中的功能切换器,从胃化到轴延伸和祖先分化.
- 检测到染色质可访问性的实质性变化,特别是在基因间区域,具有差异性的转录因子足迹,基本上独立于视网膜酸.
结论:
- 为控制脊椎动物胚胎发育中向轴延伸过渡的监管转变提供了全面的视图.
- 揭示了细胞环境调节特定生物结果的调节因子活动的机制.
- 表明存在冗余增强剂,确保在发育过程中强大的基因表达模式.
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