NCAPG通过有氧糖溶解刺激肺腺癌细胞干细胞
Zuwang Zhang1, Dongdong Qi1, Xun Liu1
1Department of Thoracic Surgery, University-Town Hospital of Chongqing Medical University, Chongqing, China.
The clinical respiratory journal
|August 9, 2023
概括
高NCAPG水平通过激活有氧糖解促进肺腺癌 (LUAD) 细胞干细胞和增殖. 用2-DG抑制糖解可以逆转这些效应,这表明NCAPG是潜在的LUAD生物标志物和治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症干细胞研究研究
背景情况:
- 癌症干细胞 (CSCs) 驱动瘤进展和肺腺癌 (LUAD) 的治疗耐药性.
- 过度表达NCAPG与瘤发生有关,但其在LUAD CSC中的作用需要研究.
- 了解NCAPG对LUAD干性的影响对于开发向疗法至关重要.
研究的目的:
- 调查NCAPG在LUAD中调节癌症干细胞特性中的作用.
- 探索NCAPG,茎性和LUAD中的糖解路径之间的关联.
- 评估NCAPG作为潜在的诊断生物标志物和LUAD治疗点.
主要方法:
- 在LUAD组织和细胞系中NCAPG表达的生物信息分析.
- 对于NCAPG表达的定量逆转录PCR (qRT-PCR) 方法.
- 细胞计数套件-8 (CCK-8)用于生命力,球形造型测试用于干性,以及干细胞标记物 (CD133,CD44,Oct-4) 和糖解酶 (HK2,PKM2,LDHA) 的西部抹杀.
- 检测糖溶性代谢物和分析细胞外酸化率 (ECAR) 和氧气消耗率 (OCR).
主要成果:
- 在LUAD中,NCAPG显著上调,并且与糖溶性标记基因正相关.
- 过度表达NCAPG增强了LUAD细胞的增殖,干细胞和糖解活性.
- 使用2-DG抑制糖解,逆转了NCAPG的前干性和增殖效应.
结论:
- 通过激活有氧糖解路径,NCAPG促进LUAD细胞干细胞和增殖.
- NCAPG代表了对LUAD诊断的潜在生物标志物.
- 准NCAPG或糖解途径可能是LUAD的可行的治疗策略.
相关概念视频
Adaptive Mechanisms in Cancer Cells
5.8K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.8K
Cancer Stem Cells and Tumor Maintenance
5.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.0K
Glycolysis
53
Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...
53
cAMP-dependent Protein Kinase Pathways
6.4K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.4K
GPCRs Regulate Adenylyl Cylase Activity
5.7K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.7K
Other Glycolytic Pathways
47
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
47


