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米色素蛋白OsHMGB1通过影响色素的可访问性,参与酸盐平衡和植物生长

Yan Wang¹, Fei Wang¹, Hong Lu¹

¹State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.

The New phytologist

|August 9, 2023

概括

米蛋白OsHMGB1通过控制无机酸盐 (Pi) 水平,有助于植物生长. 它通过对酸盐饥饿反应基因的表观遗传调节来增强Pi稳态和植物发育.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
植物生理学植物生理学

背景情况:

对于植物生长至关重要,但其信号通路,特别是表观遗传调节,尚不清楚.
无机酸盐 (Pi) 是植物发育的关键营养素.

研究的目的:

研究大米 (Oryza sativa) 中高流动性组盒1蛋白 (OsHMGB1) 的功能和机制.
阐明OsHMGB1在调节无机酸盐 (Pi) 稳态和植物生长中的作用.

主要方法:

在大米中的分子和遗传方法.
突变和过度表达线的表型分析.
转录基因组深层测序 (RNA-Seq).
染色体免疫沉,然后进行测序 (ChIP-Seq).
检测转化酶可访问的染色质,然后进行测序 (ATAC-Seq).

主要成果:

在Pi饥饿下,OsHMGB1的表达增加,蛋白质定位到核中.
OsHMGB1积极调节Pi的稳态和植物生长.
OsHMGB1与酸盐饥饿反应 (PSR) 基因的促进者结合,调节它们的表达.
OsHMGB1保持了染色质的可访问性,与基因表达相关.

结论:

OsHMGB1 作为大米中的转录促进剂.
它通过增加染色质可访问性来调节PSR基因以维持Pi平衡.
这揭示了植物适应Pi限制条件的关键表观遗传机制.

关键词:

米树 (Oryza sativa) L. 米树 (Oryza sativa) L. 米树 (Oryza sativa) 是一个植物.染色质可访问性染色质可访问性基因表达的基因表达方式高流动性组蛋白质的高流动性组蛋白质.是一种的物质.

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