培养器呈现温度分级,用于细胞的耐热度估计
Chikahiro Imashiro1,2, Yangyan Jin3, Motoaki Hayama3
1Graduate School of Engineering, The University of Tokyo, Tokyo 113-0033, Japan.
Cyborg and bionic systems (Washington, D.C.)
|August 9, 2023
概括
这项研究引入了一种新的细胞培养系统,用于高温症研究的温度分级. 该系统有助于通过评估细胞对不同温度的反应来研究癌症治疗的最佳热条件.
科学领域:
- 生物医学工程 生物医学工程
- 癌症研究 癌症研究
- 细胞生物学 细胞生物学
背景情况:
- 超热是一种有前途的非侵入性癌症治疗方法,利用癌细胞的热不耐受性.
- 精确的温度调节对于调查热细胞毒性和优化高温症协议至关重要.
- 由于需要对不同类型的细胞进行多次实验和精确的温度控制,现有的方法面临局限性.
研究的目的:
- 开发一种先进的细胞培养系统,使金属表面的温度可以精确分级.
- 为了促进对各种细胞类型的热细胞毒性进行全面调查.
- 为了克服超热研究中多个实验的瓶.
主要方法:
- 开发一种细胞培养系统,使用金属培养表面,控制温度分级.
- 使用密歇根癌症基金会-7 (MCF-7) 细胞作为癌症模型和正常人皮肤纤维细胞作为正常细胞模型.
- 根据开发的系统,评估癌症和正常细胞之间的差异性热耐受性.
主要成果:
- 这种新系统显示了即时的温度分级,允许同时评估不同温度下的细胞反应.
- 与癌细胞相比,正常细胞表现出更大的耐热性.
- 该系统有效地促进了对高温症的最佳热条件的调查.
结论:
- 开发的温度梯度细胞培养系统有效地研究了最佳的高热条件.
- 这项技术可以有效地评估细胞对热刺激的反应,为进一步研究热癌症治疗铺平了道路.
- 该系统解决了以前方法的局限性,加速了有效的高温治疗方法的开发.
相关概念视频
01:13Equipments Used to Measure Body Temperature
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
01:14Assessing Body Temperature - Oral
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
01:27Assessing Body Temperature - Rectal
Rectal temperature measurement is considered the most precise method for assessing core body temperature and typically registers higher than oral temperature. For adults, the rectal thermometer should be inserted 1 to 1.5 inches into the rectum to obtain the most accurate reading.
Follow these steps for rectal temperature assessment:
Step 1: Perform hand hygiene and don clean gloves to prevent cross-infection.
Step 2: Position the patient in a side-lying position to better visualize the rectal...
Follow these steps for rectal temperature assessment:
Step 1: Perform hand hygiene and don clean gloves to prevent cross-infection.
Step 2: Position the patient in a side-lying position to better visualize the rectal...