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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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X-ray Crystallography02:18

X-ray Crystallography

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The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
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X-ray Imaging01:24

X-ray Imaging

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German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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相关实验视频

Updated: Jul 19, 2025

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
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用CITIUS充电整合探测器进行布拉格连贯衍射成像.

Michael Grimes1,2, Kristof Pauwels2, Tobias U Schülli2

  • 1Université Grenoble Alpes, CEA Grenoble, IRIG, MEM, NRS, 17 rue des Martyrs, F-38000 Grenoble, France.

Journal of applied crystallography
|August 9, 2023
PubMed
概括

新的高速X射线成像仪CITIUS探测器显著加速了布拉格连贯衍射成像. 它实现了与光子计数探测器相似的分辨率,但是在很短的时间内.

关键词:
布拉格连贯衍射成像的成像充电集成探测器的检测器动态范围的动态范围.第四代同步电子是什么?用光子计数检测器进行检测.

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Direct Imaging of Laser-driven Ultrafast Molecular Rotation
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Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

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相关实验视频

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科学领域:

  • 在X射线成像中使用X射线成像.
  • 衍射成像技术的成像
  • 材料科学是一种材料科学.

背景情况:

  • 高速X射线成像对于先进的材料分析至关重要.
  • 布拉格连贯衍射成像 (BCDI) 需要具有高动态范围和速度的探测器.
  • 现有的探测器在采集速度和数据吞吐量方面面临限制.

研究的目的:

  • 为了评估CITIUS探测器在BCDI应用中的性能.
  • 将CITIUS探测器与BCDI现有的光子计数探测器进行比较.
  • 评估CITIUS在动态BCDI测量方面的潜力.

主要方法:

  • 在欧洲同步仪极其明亮源 (ESRF-EBS) 中使用了CITIUS探测器.
  • 在纳米级材料上进行BCDI实验.
  • 与光子计数探测器比较空间分辨率和采集时间.

主要成果:

  • CITIUS探测器实现了20 ± 6nm的空间分辨率,相当于光子计数探测器 (22 ± 9nm).
  • 使用CITIUS,获取时间从200秒大幅缩短到23秒.
  • 在高率 (17.4 kHz) 和高和度计数率 (>30 Mcps/像素) 上显示一致的线性响应.

结论:

  • 该CITIUS探测器为BCDI提供了显著的进步,使得更快,更有效的成像.
  • 它的高速和线性响应使其适合动态BCDI实验.
  • 建议进一步优化以提高其用于先进连贯成像技术的能力.