Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
Flame Photometry: Overview01:02

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
Statistical Package for the Social Sciences (SPSS)01:22

Statistical Package for the Social Sciences (SPSS)

The Statistical Package for the Social Sciences, or SPSS, is a data management and analysis software suite. Developed by SPSS Inc. in 1968 and acquired by IBM in 2009, this tool was initially designed for social science data analysis, evolving to serve a wider range of disciplines. It was later renamed to Statistical Product and Service Solutions.
SPSS streamlines the process from data preparation to analysis and reporting. It is characterized by its user-friendly interface, which conceals...
Statgraphics01:10

Statgraphics

Statgraphics is a comprehensive statistical software suite designed for both basic and advanced data analysis. Originating in 1980 at Princeton University under Dr. Neil W. Polhemus, it was one of the pioneering tools for statistical computing on personal computers, with its public release in 1982 marking an early milestone in data science software. Over the years, it has evolved into a robust platform for data science, offering tools for regression analysis, ANOVA, multivariate statistics,...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mitoquinone Prevents Cardiac Dysfunction by Normalizing Mitochondrial ROS and Calcium Handling in Acute Myocardial Infarction.

Acta physiologica (Oxford, England)·2026
Same author

Ca<sub>V</sub>1.2 dynamics in native male arterial myocytes.

The Journal of physiology·2026
Same author

The ultrarapid delayed rectifier potassium current has important functional role in the repolarization reserve of canine and human ventricular muscle.

The Journal of physiology·2026
Same author

Tirzepatide Regulates Pacemaker Function by Modulating cAMP and Calcium Dynamics in Human Sinoatrial Node Cells.

Circulation·2026
Same author

Mitochondrial protein OPA3 sustains cardiac function by regulating calcium handling in male mice.

Nature communications·2026
Same author

Targeting Arrhythmogenic Late Sodium Current by FHF1<sub>A</sub>-Derivative FixR in Heart Failure Cardiomyocytes.

Circulation research·2026

相关实验视频

Updated: Jun 27, 2026

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
11:22

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

Published on: February 24, 2014

15.3K

SparkMaster 2:一种用于自动分析火花数据的新软件.

Jakub Tomek1,2, Madeline Nieves-Cintron1, Manuel F Navedo1

  • 1Department of Pharmacology, University of California, Davis School of Medicine, Davis, California (J.T., M.N.-C., M.F.N., C.Y.K., D.M.B.).

Circulation research
|August 9, 2023
PubMed
概括

SparkMaster 2 (SM2) 是一种新的,用户友好的软件,用于分析细胞成像中的 (Ca) 火花. 它准确地识别了Ca释放事件,改善了用于细胞功能障碍研究的高吞吐量分析.

关键词:
节律失常可能是心律失常.信号传递的.肌细胞,心脏细胞.软件 软件 软件 软件 软件

更多相关视频

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences
06:49

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

Published on: June 16, 2014

17.2K
Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

9.3K

相关实验视频

Last Updated: Jun 27, 2026

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
11:22

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

Published on: February 24, 2014

15.3K
Automated Analysis of Dynamic Ca2+ Signals in Image Sequences
06:49

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences

Published on: June 16, 2014

17.2K
Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

9.3K

科学领域:

  • 细胞生理学 细胞生理学
  • 生物物理学的生物物理.
  • 医学成像分析 医学成像分析

背景情况:

  • (Ca) 的火花对于细胞的Ca释放至关重要,特别是在心肌细胞中.
  • 火花成像对于了解在健康和疾病中的处理,包括心律失常,至关重要.
  • 由于大量的实验体积,对Ca成像数据的高通量分析至关重要.

研究的目的:

  • 开发并介绍SparkMaster 2 (SM2),这是一款用于分析Ca火花成像数据的新型软件.
  • 提供一个用户友好,准确和灵活的工具,用于高吞吐量分析Ca释放事件.
  • 改进Ca火花和相关现象的识别和表征.

主要方法:

  • 开发SparkMaster 2 (SM2) 软件的开发工作.
  • SM2是一个独立的应用程序,具有图形用户界面和Python脚本功能.
  • 通过共聚焦线扫描显微镜成像的Ca火花的分析.

主要成果:

  • SM2在识别Ca释放事件方面表现出高精度,超过了以前的软件.
  • SM2可以区分和分析各种Ca释放事件,包括火花,波和迷你波.
  • SM2准确地分析了集群中的单个火花,并且适用于不同的细胞类型和成像条件.

结论:

  • SparkMaster 2 (SM2) 是一个改进的,用户友好的软件,用于准确,高吞吐量分析Ca火花成像.
  • SM2促进了Ca火花数据的可视化,分析和解释,提高了研究质量.
  • 预计SM2将推进对心肌细胞和其他细胞类型中非节律性Ca释放事件的研究.