合成肝酶抑制剂可以预防简单疹病毒的传播
Pradeep Chopra1, Tejabhiram Yadavalli2, Francesco Palmieri3
1Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
Angewandte Chemie (International ed. in English)
|August 9, 2023
概括
通过使用HS-oligosaccharides抑制人类肝酶 (Hpse),通过防止HS脱落来阻止单纯疹病毒1型 (HSV-1) 的传播. 这为HSV-1感染提供了一种新的治疗策略.
科学领域:
- 病毒学 病毒学
- 生物化学 生物化学
- 药物发现 药物发现 药物发现
背景情况:
- 简单疹病毒1型 (HSV-1) 使用硫酸肝素 (HS) 进行细胞附着和进入.
- 在感染期间,HSV-1上调人类肝酶 (Hpse) 降解HS,促进病毒传播.
研究的目的:
- 研究HPse抑制作为HSV-1的治疗策略.
- 探索HS-寡糖的结构-活性关系在抑制Hpse.
主要方法:
- 各种HS-寡糖的合成.
- 酶抑制试验用于评估Hpse活性.
- 计算建模以了解结构-活动关系.
- 人类角膜上皮细胞 (HCE) 感染HSV-1并用HS-oligosaccharides进行治疗.
主要成果:
- HS的六糖和八糖化合物强烈抑制了Hpse.
- 发现,对Hpse抑制而言,二氧硫化的氨酸被容忍.
- HS-oligosaccharide治疗阻断了感染HCE的HSV-1诱导的HS分泌,减少了病毒的传播.
- 这些化合物还抑制了HCE的迁移和扩散.
结论:
- 使用特定的HS-oligosaccharides抑制hpse是一种对HSV-1的有希望的治疗方法.
- 该研究阐明了有效抑制Hpse的结构要求.
- HS-寡糖体通过减少病毒传播和抑制细胞增殖,显示出双重治疗潜力.
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