相关实验视频

Updated: Jul 19, 2025

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
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使用TIRF显微镜的光学子来量化植物内膜系统中器官之间的物理相互作用

Eleanor M Fletcher1, Benji C Bateman2, Stanley W Botchway2

  • 1School of Biological Sciences, University of Bristol, Bristol, United Kingdom.

Current protocols
|August 9, 2023
PubMed
概括

研究人员使用TIRF光学子开发了一种方法,用于量化膜接触部位的植物器官相互作用. 这种技术可以对参与有机细胞结合的结合蛋白进行可重复和可重复的测试.

关键词:
领地 领地 领地细胞内膜网膜的内oplasmic网膜.光学子,一个光学子.植物有机体 植物有机体

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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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