沙门氏菌YqiC通过一个寡合状态来发挥其功能
Wei-Chun Huang1, Wai-Ting Chen1, Yueh-Chen Chen1
1Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Protein science : a publication of the Protein Society
|August 9, 2023
概括
沙门氏菌 Typhimurium 是一个类型.
科学领域:
- 微生物学 微生物学
- 蛋白质生物化学 蛋白质生物化学
- 细菌病原体的产生
背景情况:
- 蛋白质寡合化影响生物功能.
- 沙门氏菌 Typhimurium 中的 YqiC 蛋白质形成一个同类三元体,与细菌殖民和入侵有关.
- 了解YqiC的寡合状态对于阐明它在感染中的作用至关重要.
研究的目的:
- 为了研究YqiC蛋白质寡合化在沙门氏菌Typhimurium病原发生的意义.
- 确定YqiC的交互伙伴及其在细菌毒性中的作用.
主要方法:
- 在YqiC的卷-卷域的位点导向突变发生.
- 使用化学交叉连接和SEC-MALS确认了三元体损失.
- 用野生型和突变型YqiC补充yqiC淘汰菌株.
- 使用拉下测试和质谱的蛋白质-蛋白质相互作用研究.
主要成果:
- 突变的关键残留物扰乱了YqiC同类三元体的形成.
- 突变YqiC未能恢复沙门氏菌的殖民化和入侵.
- YqiC与电子运输链复合II子单元 (SdhA,SdhB) 和F0F1-ATP合成酶β子单元相互作用.
- 确定的相互作用表明YqiC调节细菌能量代谢和毒性因子组合.
结论:
- 对于沙门氏菌Typhimurium的毒性,YqiC的寡合化是必不可少的.
- YqiC与代谢酶的相互作用会影响细菌的病变发生.
- YqiC代表了对抗沙门氏菌感染的潜在治疗目标.
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