相互测试交叉设计用于全基因组预测玉米单交叉性能
Patrick K Sweet1, Rex Bernardo2
1Syngenta Seeds, Stanton, MN, 317 MN-19, USA.
概括
在玉米育种中,相互交叉测试设计通过增加交叉测试之间的相关性来提高全基因组预测的准确性. 这种方法证明优于预测产量和水分的传统设计,有助于开发改进的玉米品种.
科学领域:
- 植物育种和遗传学 植物育种和遗传学
- 基因组学和生物信息学
- 农业科学 农业科学
背景情况:
- 玉米 (Zea mays L.) 的杂交发展依赖于有效预测繁殖价值.
- 传统的交叉测试设计可能无法完全捕捉最佳基因组范围预测所需的遗传相关性.
- 相互测试交叉设计通过战略性地利用来自对立异性群体的父母杂交作为测试者来提供潜在的改进.
研究的目的:
- 评估相对测试交叉设计的优越性与传统的非相对设计相比,用于玉米的全基因组预测.
- 评估相互测试交叉对产量和湿度特征预测能力的影响.
- 为了确定相互设计中增加的相关性是否提高了预测的有效性.
主要方法:
- 从10个玉米繁殖种群中发展出双倍的平分体.
- 采用相互测试交叉设计,交叉双倍哈普洛伊德与来自对立异性群体的测试者.
- 在多位置产量试验中评估了1642个测试交叉,并使用单核酸多态度标记器的全基因组预测分析了数据.
主要成果:
- 与传统设计相比,相互测试交叉设计的产量/湿度和湿度预测能力比传统设计高0.11至0.26.
- 基于相互设计预测的不同选择导致所有种群的产量/水分和水分显著增加.
- 对于产量,没有观察到预测能力的显著差异,相反的设计显示出这种特性的预测能力较低.
结论:
- 相互交叉测试设计比传统设计更有效,用于在玉米中进行全基因组预测,特别是对于水分和产量/水分特征.
- 增强的预测能力归因于培训和测试群体之间的相关性增加.
- 相互测试交叉是一种有价值的策略,可以改善近亲血统的发展,并加速玉米育种计划中的遗传收益.
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