切割的叶子1编码了一个MYB转录因子,控制了土豆中的叶子形态
Dawei Li1, Xiaoyue Lu1, Duoduo Qian1,2,3
1Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
概括
转录因子StDL1控制了土豆叶的剖析. 一种衰退的Stdl1/Stdl1基因型,导致非切割的叶子,在种植的土豆中变得越来越常见,影响了植物形态.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 叶子形态对于植物结构,光合作用和产量至关重要.
- 马 (Solanum tuberosum L.) 呈现出各种各样的叶形状,但遗传调节剂的了解很少.
- 已经确定了控制土豆叶子发育的少数基因.
研究的目的:
- 为了确定调节土豆中切割的叶子形成的基因.
- 研究StDL1基因在土豆叶子发育中的作用.
- 为了分析种植土豆种群中StDL1等位基因的频率.
主要方法:
- 对R2R3 MYB转录因子的鉴定,切割叶1 (StDL1).
- 对自然存在的 Stdl1 基因组进行分析,该基因组赋予未经切割的叶子.
- 在双胞胎土豆中的StDL1淘汰实验以及在N. benthamiana和酵母中的功能测试.
- 对373个土豆加入的基因定型,以确定Stdl1/Stdl1频率.
主要成果:
- 鉴定出StDL1是土豆中切割的叶子形成的关键调节者.
- 衰退的Stdl1等位基因导致年轻苗木中的非切割的叶子.
- StDL1作为一个转录激活剂.
- 在种植的土豆中,衰退型Stdl1/Stdl1基因型的频率显著增加.
结论:
- 在土豆中,StDL1是切割叶子形态的关键遗传决定因素.
- 在土豆种植中,Stdl1/Stdl1基因型的流行率增加表明对非切割的叶子进行了选择.
- 这项研究阐明了土豆叶形状的遗传基础,并提供了对其演变的见解.
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