Rege-1 通过调节 IIS 和 TOR 途径来促进 C. elegans 的生存
Yi-Ting Tsai1, Chen-Hsi Chang2, Hsin-Yue Tsai1,3
1Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
PLoS genetics
|August 9, 2023
概括
核糖核酶 REGE-1 通过调节 ets-4 mRNA 来控制 C. elegans 的生存. 失去REGE-1功能导致过早死亡,与代谢途径失调有关.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 代谢途径调节对环境线索的生理反应.
- 严格控制这些反应对于生物体的恒温是必不可少的.
- 核糖核酶REGE-1在应激反应中的作用尚未完全理解.
研究的目的:
- 为了研究REGE-1核糖酶在Caenorhabditis elegans中的功能.
- 阐明REGE-1调节病原体生存的分子机制.
- 为了确定由REGE-1活动影响的代谢途径.
主要方法:
- 凯诺拉布迪斯的应激反应测试.
- 用于全球基因表达分析的RNA测序 (mRNA-seq).
- 染色体免疫沉 (ChIP) 数据分析和基因删除研究.
主要成果:
- 缺陷的REGE-1核核糖酶活性导致C. elegans在感染Pseudomonas aeruginosa时过早死亡,这取决于转录因子ets-4.
- REGE-1 调节胰岛素/IGF 信号传递 (IIS) 和拉巴胺素 (TOR) 激酶通路的标.
- 在rege-1突变体中ets-4mRNA降解的失调导致II类长寿基因的上调和TORC1信号的激活,导致生存率低下.
结论:
- REGE-1对于C. elegans的生存至关重要,它通过严格调节对环境刺激的生理反应,特别是对病原体的暴露.
- REGE-1 控制 ets-4 mRNA 和下游代谢途径 (包括 IIS 和 TOR) 的功能对于预防过早死亡至关重要.
- 这些发现突出了REGE-1在调节应激反应和代谢信号的保护作用,类似于其哺乳动物正方体Regnase-1.
相关概念视频
Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Regulated Protein Degradation
7.4K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.4K


