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带有大型伪斯托克斯转移的电比度光传感器,用于精确的pH传感和成像,没有干扰的背景光

Tao Wang¹, Chuan Liao², Zike Jiang¹

¹Institute of Oceanographic Instrumentation, Qilu University of Technology (Shandong Academy of Sciences), Shandong Provincial Key Laboratory of Marine Monitoring Instrument Equipment Technology, National Engineering and Technological Research Center of Marine Monitoring Equipment, Qingdao 266061, PR China.

|August 9, 2023

概括

研究人员开发了新的化物纳米颗粒 (LFNP),具有很大的伪斯托克斯转移,用于无背景光pH传感. 这一进步使复杂的生物样本能够通过最小化干扰来进行准确的pH测量和成像.

科学领域:

材料科学材料科学材料科学
纳米技术纳米技术
分析化学分析化学

背景情况:

光pH传感器经常受到背景干扰,这是由于重叠的辐射光谱造成的.
开发具有较大的斯托克斯转移的发光材料对于克服这一局限性至关重要.
在光传感器中实现大斯托克斯移位的现有方法是有限的.

研究的目的:

设计和合成化物纳米颗粒 (LFNP) 化物化物化物 (LFNP) 具有非常大的伪斯托克斯转移.
开发一种新的光pH传感器系统,利用这些LFNP进行准确和无背景pH测量.
为了证明这种传感器系统在真实生物样本中的应用.

主要方法:

制造NaGdF4:Ce@NaGdF4:Nd@NaYF4:Eu双核外 (CDS) 化物纳米颗粒 (LFNP) 化物化物化物化物.
利用Gd3+介导的能量迁移和界面能量转移来产生强烈的红色和NIR辐射.
集成CDS LFNPs与绿色,以创建光传感器膜,并使用双芯片RGB-NIR摄像头进行信号检测.

主要成果:

在254nm辐射下,取得了361nm (红色辐射) 和610nm (NIR辐射) 的显著伪斯托克斯移.
根据内部波器效应和强度比,开发了一种具有5-6 (pKa ± 0.5) 范围的比度pH传感器.
在像藻类和血清这样的自身光真实样本中证明了精确的pH测定,具有高的信号比噪声比.

结论:

开发的CDS LFNP提供了一个有前途的平台,用于创建具有大型伪斯托克斯转移的光传感器.
新的pH传感器系统通过将排放信号与背景光分离,使得无背景测量和2D成像成为可能.
这种方法为复杂和自发光环境中精确的pH测定提供了新的策略.

关键词:

没有背景的pH感应.内部波器效应是什么?化物纳米颗粒添加了兰化物.大型伪斯托克斯转移.在RGB-NIR成像中使用RGB-NIR成像.电比测量光传感器传感器

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