新加坡小虹膜病毒VP122针对小STING,以逃避干扰素的免疫反应
Linting Xu1, Mengke Liu1, Hong Chen1
1College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Fish & shellfish immunology
|August 9, 2023
概括
新加坡群虹膜病毒VP122蛋白通过向EcSTING来抑制cGAS-STING抗病毒途径. 这种相互作用破坏了免疫基因转录,帮助SGIV复制和宿主逃避.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 新加坡毛虹膜病毒 (SGIV) 是一种引起毛疾病的重要病原体.
- 许多SGIV开放式读取框架 (ORF) 缺乏功能特征,阻碍了对病毒机制的理解.
研究的目的:
- 为了描述SGIV ORF122的功能,它被指定为VP122.
- 研究VP122在病毒复制和宿主免疫反应调节中的作用.
主要方法:
- 在GS细胞中过度表达VP122.
- 免疫基因转录 (IFN1,IFN2,ISG15,ISG56,PKR,TNF-α) 的分析.
- 对cGAS-STING通路激活,ISRE促进体活性,蛋白质-蛋白质相互作用 (共免疫沉降,西斑) 的测试.
主要成果:
- VP122过度表达增强了SGIV复制.
- VP122抑制了cGAS-STING介导的免疫基因转录和ISRE促进体激活.
- VP122与EcSTING直接相互作用,抑制了它的自我相互作用,但没有影响EcTBK1/EcIRF3的招募.
结论:
- SGIV VP122通过准 EcSTING 来对抗宿主I型干扰素反应.
- 在SGIV的免疫逃避策略和宿主病毒相互作用中,VP122起着至关重要的作用.
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