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来自地下土壤的新型BTEX降解菌株:隔离,鉴定和生长评估

Gurpreet Kaur¹, Joanna Lecka², Magdalena Krol¹

¹Department of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, Ontario, M3J 1P3, Canada.

Environmental pollution (Barking, Essex : 1987)

|August 9, 2023

概括

地热加热可以增强有毒,,乙和 (BTEX) 污染物的生物修复. 地下土壤的变暖促进了这些致癌化合物的微生物降解.

科学领域:

环境微生物学环境微生物学
生物修复技术生物修复技术
地热能源应用地热能源应用

背景情况:

诸如,,乙烯和西 (BTEX) 等单芳香碳化合物是加拿大污染场所发现的致癌污染物.
低地表温度 (5-10°C) 抑制了内在微生物群落的自然生物修复能力.
需要有效的策略来克服温度限制,以便在现场降解污染物.

研究的目的:

调查使用地热加热来增强BTEX在地下环境中的生物修复的可行性.
从各种土壤深度中分离和识别新的BTEX降解细菌菌株.
评估温度升高对微生物生长和BTEX降解率的影响.

主要方法:

从不同深度 (3.0,42.6,73.2米) 的土壤样本中分离和选强大的BTEX降解细菌.
使用桑格测序和植物遗传学分析识别细菌分离物.
实验以确定不同温度 (15°C,28°C,40°C) 对生物降解效率的影响.

主要成果:

包括Microbacterium esteraromaticum和Bacillus infantis在内的新型细菌菌株显示出显著的BTEX降解能力.
在最佳条件下,乙基的降解效率高达77.52%,烯的降解效率高达74.58%.
温度升高 (28°C和40°C) 导致微生物生长增加了两倍,生物降解增加了35-49%.

结论:

温度是BTEX生物修复的关键积极刺激剂.
使用地热能增加地表热量显示出有很大的潜力,可以增强BTEX污染地区的现场生物修复.
这种方法为管理危险芳香碳化合物污染提供了一个有希望的可持续解决方案.

关键词:

在 BTEX 中使用 BTEX.细菌细菌是一种细菌.生物降解生物降解隔离隔离隔离隔离土壤土壤土壤.温度温度是指温度.

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