在膀癌中,RAB14通过自性依赖AKT信号通路促进表皮-介质细胞过渡
Huanhuan Deng1, Leihong Deng2, Haichao Chao3
1Medical College of Nanchang University, Nanchang, Jiangxi, 330006, China.
Cell death discovery
|August 9, 2023
概括
与自相关的基因RAB14通过增强表皮细胞转移到介质细胞转移 (EMT) 和自促进膀癌 (BLCA) 侵袭和转移. 抑制RAB14可能为BLCA患者提供治疗策略.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 分子医学是分子医学.
背景情况:
- 膀癌 (BLCA) 是癌症死亡的一个重要原因.
- 自和上皮转介质转变 (EMT) 在癌症转移中至关重要,但它们在BLCA中的相互作用仍然不清楚.
研究的目的:
- 研究与自相关基因在BLCA进展中的作用.
- 阐明自,EMT和膀癌转移之间的关系.
- 为了确定BLCA的潜在治疗点.
主要方法:
- 功能丰富和途径网络分析被用来识别候选自相关基因.
- 蛋白与蛋白相互作用 (PPI) 网络是为了优先考虑基因而构建的.
- 在TCGA数据集和细胞系 (T24) 中分析了RAB14表达.
- 功能测定 (迁移,入侵,EMT,自) 和分子分析 (免疫光学,电子显微镜,西部涂抹,IHC) 在RAB14敲击后进行.
主要成果:
- RAB14被确定为一个关键的自相关基因,在BLCA中持续升调,并与不良的临床结果相关.
- 敲除RAB14显著抑制了EMT,迁移和T24细胞的入侵.
- 拉巴14与自标志物 (LC3B,Beclin1) 呈正相关性,其对EMT和转移的作用被拉巴素部分逆转.
- RAB14对自的影响与PI3K/AKT信号通路有关.
结论:
- 与自相关的基因RAB14促进膀癌细胞的迁移,入侵和EMT.
- RAB14通过与Akt相关的自途径促进这些过程.
- RAB14代表了抑制膀癌进展的潜在治疗标.
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