孕激素和雄激素的发生和转化在陆地植物中得到保护
Glendis Shiko1, Max-Jonas Paulmann1, Felix Feistel2
1Department of Plant Physiology, Matthias-Schleiden-Institute for Genetics, Bioinformatics and Molecular Botany, Friedrich Schiller University Jena, Jena, 07743, Germany.
The New phytologist
|August 10, 2023
概括
植物的孕激素和雄激素在整个绿色血统中被保存,并观察到关键的转化. 它们的生物合成似乎有利于 Δ4 途径,特别是在单种植物中.
科学领域:
- 植物生物化学 植物生物化学
- 类固醇生成的演变.
- 植物菌体分析
背景情况:
- 孕激素和雄激素存在于植物中,但它们的生物合成和进化起源在很大程度上仍未被描述.
- 了解植物中的类固醇生成对于理解植物进化和潜在应用至关重要.
研究的目的:
- 为了研究孕激素和雄激素在Viridiplantae系的保存和生物合成.
- 识别关键的植物类固醇及其代谢转化.
- 为了探索绿色植物中类固醇生成的进化方面.
主要方法:
- 采用超高性能液态染色学 - - 电子喷雾电离 - - 双重质谱法 (UHPLC-ESI-MS/MS) 进行高通量分析.
- 分析了41种跨越绿色植物谱系的物种.
- 量化了孕激素和雄激素,并追踪了它们的化学转化.
主要成果:
- 孕激素和雄激素在维里迪植物谱系中得到保护.
- 脱氨,和5α-二氨被确定为植物中大量存在的类似哺乳动物的类固醇.
- 观察到孕激素的转化为17α-基孕激素和5α-pregnane-3,20-dione.
- 观察到雄激素转化为和5α-二二.
- 17,20-酶对于孕激素转化为雌激素的关键作用,在单种动物中显示出更高的疗效.
结论:
- 孕激素和雄激素的存在在植物中高度保留.
- 植物类固醇生物合成可能有利于Δ4通路.
- 17,20-酶的酶效率在不同植物群体之间可能有所不同,单种植物的效率更高.
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