胸膜上皮细胞在早期器官生成中的命运和功效,通过单细胞转录和功能分析进行评估
Alison Mary Farley1, An Chengrui1, Sam Palmer2
1Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
Frontiers in immunology
|August 10, 2023
概括
早期的甲状腺发育显示出不同的原始细胞,而不是一种常见的类型. 胎儿胸膜上皮原生细胞 (TEPC) 显示出可塑性,挑战现有模型并影响干细胞研究.
科学领域:
- 发展生物学 发展生物学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 皮层 (cTEC) 和骨髓 (mTEC) 胸膜上皮细胞 (TEC) 起源于第三喉囊内皮.
- 现有的模型提出,小鼠的常见胸膜上皮原生细胞 (TEPC) 到胚胎12.5天 (E12.5) 的状态,能够产生cTEC和mTEC.
研究的目的:
- 为了研究TEC在早期胆乳腺中的命运和功效.
- 挑战在E12.5胸腺中占主导地位的常见TEPC的主导模式.
主要方法:
- 单细胞RNA测序 (scRNAseq) 用于识别祖先种群.
- 谱系追踪实验,以验证祖细胞命运.
- 实力分析,以评估TEC的差异化潜力.
主要成果:
- 在E12.5确定了一个候选mTEC祖先种群,并通过谱系追踪证实mTEC的命运受限.
- 大多数E11.5和E12.5原始TEC被发现是cTEC命运.
- 在大多数E12.5 cTEC注定 TEPC中,夜间培养诱导了功能性双能,并确定了潜在的分子机制.
结论:
- E12.5 胸腺含有亚系原始的祖先,没有一个共同的 TEPC.
- 早期胎儿的TEPC表现出受外部因素影响的细胞酸盐可塑性.
- 这些发现有助于更好地了解胎儿胸膜上皮的发育,并对从多能干细胞生成TEC产生影响.
关键词:
细胞的命运 细胞的命运细胞身份是细胞的身份.细胞状态可塑性 细胞状态可塑性皮质细胞是表皮细胞.强度强度强度是什么意思一个单细胞的单细胞.胸腺 (thymus) 是一个神经系统.转录组 (transcriptome) 是一个转录组.更多相关视频
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