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黄囊膜中的葡萄糖生成:在层发育期间的酶活性,基因表达和代谢物

Mitsuhiro Shibata¹, Atsushi Iwasawa², Masato Yayota²

¹The United Graduate School of Agricultural Science, Gifu University, Gifu 501-1193, Japan.

The journal of poultry science

|August 10, 2023

概括

黄囊膜是开发小的关键葡萄糖生成器官,在早期化期间从乳酸中积极产生葡萄糖,并在以后利用黄囊脂质. 它的酶活性随着发育阶段的变化而改变,以满足胚胎的需求.

科学领域:

* 生物化学和分子生物学
* 发育生理学发展生理学
* 鸟类胚胎学鸟类胚胎学

背景情况:

* 葡萄糖生成对胚胎发育至关重要,在饮食摄入量不足时提供葡萄糖.
*黄囊膜在鸟类胚胎葡萄糖生成中的作用尚未完全理解.
*了解这些代谢途径对于优化家禽生产和胚胎生存能力至关重要.

研究的目的:

* 在蛋化过程中调查黄囊膜中关键葡萄糖生成酶的mRNA表达和酶活性.
* 量化黄囊膜中的代谢基质 (乳酸,甘油三,脂肪酸,糖原,葡萄糖) 和血糖水平.
*阐明葡萄糖生成的动态变化及其在胚胎发育期间对能量代谢的贡献.

主要方法:

*每天从化日3到化后的第3天收集黄囊膜 (n=7-9).
*对葡萄糖-6-酸酶,果糖-1,6-双酸酶,酸酸碳酸酶 (细胞质和线粒体) 和酸碳酸酶的mRNA表达和酶活性进行量化.
* 测量乳酸,甘油三,非化脂肪酸,糖原和葡萄糖在黄囊膜和血糖水平.

主要成果:

*所有关键的葡萄糖生成酶都显示出可检测的mRNA表达和活性,根据发育阶段而异.
*高葡萄糖生成酶活性和早期化期间的乳酸水平表明活性乳酸葡萄糖生成,可能与早期缺氧有关.
*从化期中期开始增加的甘油三和非化脂肪酸表明黄黄脂利用为能量和葡萄糖生成,在化后期转向糖原动员和葡萄糖分泌.

结论:

* 蛋黄囊膜在蛋化过程中起到重要的葡萄糖生成器官的作用.
* 葡萄糖生成活性动态地适应胚胎不断变化的代谢需求和氧气供应.
* 黄囊膜在为胚胎发育和化提供能量基质方面发挥着至关重要的作用.

关键词:

没有PEPCK,没有PEPCK.的胚胎的胚胎葡萄糖的产生是葡萄糖的产生.乳酸乳酸是一种乳酸.黄体囊中的黄体囊

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