评估逆转录-聚合酶链反应和同时放大和测试用于定量检测血清乙型肝炎病毒RNA
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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
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Xiaohan Hu1, Liwei Zhao1, Mingrong Ou2
1Department of Laboratory Medicine, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, China.
Heliyon
|August 10, 2023
概括
同时放大和测试 (SAT) 和RT-qPCR可靠地检测乙型肝炎病毒RNA. 这些方法显示出良好的一致性,使它们可以互换用于监测慢性HBV感染中的ccccDNA转录活性.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 病毒学 病毒学
- 分子诊断学 分子诊断学
背景情况:
- 慢性乙型肝炎病毒 (HBV) 感染是一个全球性的健康问题.
- 乙型肝炎病毒的共价封闭圆形DNA (cccDNA) 是持久的复制模板,难以根除.
- 肝炎病毒RNA作为cccDNA转录活性在肝细胞中的替代标记.
研究的目的:
- 为了验证 HBV RNA 检测的同时放大和测试 (SAT) 方法的性能.
- 为了比较SAT和RT-qPCR用于定量HBVRNA的临床有效性,相关性和一致性.
主要方法:
- 对HBVRNA的SAT检测方法的性能评估.
- 通过SAT和RT-qPCR检测到的HBVRNA水平的比较分析.
- 评估SAT和RT-qPCR结果之间的相关性和一致性.
主要成果:
- 通过SAT方法,检测极限为50副本/毫升,线性范围为1x10^2-1x10^8副本/毫升.
- 通过SAT和RT-qPCR检测到的HBVRNA水平没有显著差异.
- 这两种方法之间发现了良好的相关性和一致性 (HBeAg阳性组的R2 = 0.7787,HBeAg阴性组的R2 = 0.8235).
结论:
- SAT方法和RT-qPCR方法在HBV RNA检测方面显示出极好的一致性.
- 无论是SAT和RT-qPCR都是可靠和可互换的方法来监测HBVRNA.
- 这些发现支持在临床环境中使用SAT作为RT-qPCR的可行替代方案.
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