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通过反应介导的双重识别策略识别和检测细胞内活性硫物种

Li Chen¹, Yue Guan¹, Siqing Zheng¹

¹School of Chemical and Environmental Engineering, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, P. R. China.

Analytical chemistry

|August 10, 2023

概括

研究人员开发了一种新型纳米传感器,可以同时检测活细胞中的硫化 (H2S) 和二氧化硫 (SO2). 这一进展有助于理解这些反应性硫物种在生理过程中的作用.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
细胞生物学细胞生物学

背景情况:

活性硫种 (RSS),包括硫化 (H2S) 和二氧化硫 (SO2),是生理过程中至关重要的信号分子.
了解H2S和SO2的精确作用受到生物系统中缺乏准确和强大的传感方法的阻碍.

研究的目的:

开发一种新型纳米传感器,用于在活细胞内同时准确检测H2S和SO2.
研究一种双重识别策略的潜力,以检测有反应性的硫种.

主要方法:

制造装有银纳米粒子 (AgNPs) 的MIL-101 (Fe) (ALM) 混合纳米传感器.
使用一种反应介导的双重识别策略,结合了表面增强拉曼光谱 (SERS) 和光 (FL) 检测.
使用ALM平台同时检测活细胞中的H2S和SO2,向线粒体.

主要成果:

该ALM纳米传感器展示了同时检测H2S和SO2的高灵敏度 (2.8×10−6μM为H2S,0.003μM为SO2).
H2S检测涉及AgNP氧化到Ag2S,降低SERS信号,而SO2检测涉及电荷转移复合体的形成,增加FL强度.
该平台在SERS和FL信号之间显示了最小的交叉声,并在外部刺激下启用了细胞内检测.

结论:

开发的ALM纳米传感器为在活细胞中同时检测H2S和SO2提供了一个强大的工具.
这一进步有助于更深入地了解RSS的生理作用.
双模传感方法为复杂的生物分析提供了高灵敏度和特异性.

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